Literature summary for 3.6.5.4 extracted from

Gawronski-Salerno, J.; Coon, J.S.; Focia, P.J.; Freymann, D.M.
X-ray structure of the T. aquaticus FtsY:GDP complex suggests functional roles for the C-terminal helix of the SRP GTPases (2007), Proteins, 66, 984-995.

Search for more literature for 3.6.5.4

Cloned(Commentary)

Cloned (Comment)	Organism
the vector pJGS3 expressing the protein FtsY NGd20, in which the N-terminal residues 2-20 of FtsY are deleted, is constructed by PCR-based site-directed mutagenesis of pTB88 for expression in Escherichia coli Rosetta-2DE3 pLysS cells	Thermus aquaticus

Crystallization (Commentary)

Crystallization (Comment)	Organism
the 2.1 A X-ray structure of FtsY from Thermus aquaticus bound to GDP is reported	Thermus aquaticus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP + H2O	Thermus aquaticus	-	GDP + phosphate	-	ir

Organism

Organism	UniProt	Comment	Textmining
Thermus aquaticus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
the FtsY NGd20 protein is purified over a HiTrap Blue column, desalted, then passed over a HiTrap SP and a HiTrap Q Sepharose column	Thermus aquaticus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	-	Thermus aquaticus	GDP + phosphate	-	ir

Subunits

Subunits	Comment	Organism
heterodimer	FtsY and Ffh assemble in a GTP-dependent manner to form a heterodimeric SRP targeting complex	Thermus aquaticus

Synonyms

Synonyms	Comment	Organism
FtsY	GTPase receptor, comprises a GTPase domain similar to Ffh	Thermus aquaticus
signal-recognition-particle GTPase	-	Thermus aquaticus
SRP GTPase	-	Thermus aquaticus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
42	-	GTPase assay	Thermus aquaticus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	GTPase assay	Thermus aquaticus

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Release 2023.1 (January 2023)