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Literature summary for 3.6.5.4 extracted from

  • Gawronski-Salerno, J.; Freymann, D.M.
    Structure of the GMPPNP-stabilized NG domain complex of the SRP GTPases Ffh and FtsY (2007), J. Struct. Biol., 158, 122-128.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
a construct of Thermus aquaticus FtsY in which the first 20 amino acids are deleted is subsequently expressed Thermus aquaticus
the NG domain of Thermus aquaticus Ffh is cloned and subsequently expressed Thermus aquaticus

Crystallization (Commentary)

Crystallization (Comment) Organism
the structure of the GMPPNP-stabilized complex of Thermus aquaticus Ffh and FtsY NG domains is determined at 1.97 A resolution Thermus aquaticus

Metals/Ions

Metals/Ions Comment Organism Structure
I-
-
Thermus aquaticus
K+
-
Thermus aquaticus
Mg2+
-
Thermus aquaticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Thermus aquaticus
-
GDP + phosphate
-
ir

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ffh NG is purified, the Ffh NG-FtsY NGd20 complex is purified using ion exchange chromatography, a Q Sepharose and a SP Sepharose column Thermus aquaticus
FtsY NGd20 is purified, the Ffh NG-FtsY NGd20 complex is purified using ion exchange chromatography, a Q Sepharose and a SP Sepharose column Thermus aquaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
Thermus aquaticus GDP + phosphate
-
ir

Subunits

Subunits Comment Organism
heterodimer Ffh-FtsY complex Thermus aquaticus

Synonyms

Synonyms Comment Organism
Ffh
-
Thermus aquaticus
FtsY
-
Thermus aquaticus
SRP GTPase
-
Thermus aquaticus