Cloned (Comment) | Organism |
---|---|
a construct of Thermus aquaticus FtsY in which the first 20 amino acids are deleted is subsequently expressed | Thermus aquaticus |
the NG domain of Thermus aquaticus Ffh is cloned and subsequently expressed | Thermus aquaticus |
Crystallization (Comment) | Organism |
---|---|
the structure of the GMPPNP-stabilized complex of Thermus aquaticus Ffh and FtsY NG domains is determined at 1.97 A resolution | Thermus aquaticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
I- | - |
Thermus aquaticus | |
K+ | - |
Thermus aquaticus | |
Mg2+ | - |
Thermus aquaticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Thermus aquaticus | - |
GDP + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus aquaticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ffh NG is purified, the Ffh NG-FtsY NGd20 complex is purified using ion exchange chromatography, a Q Sepharose and a SP Sepharose column | Thermus aquaticus |
FtsY NGd20 is purified, the Ffh NG-FtsY NGd20 complex is purified using ion exchange chromatography, a Q Sepharose and a SP Sepharose column | Thermus aquaticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Thermus aquaticus | GDP + phosphate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
heterodimer | Ffh-FtsY complex | Thermus aquaticus |
Synonyms | Comment | Organism |
---|---|---|
Ffh | - |
Thermus aquaticus |
FtsY | - |
Thermus aquaticus |
SRP GTPase | - |
Thermus aquaticus |