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Literature summary for 3.6.5.4 extracted from

  • Lu, Y.; Qi, H.Y.; Hyndman, J.B.; Ulbrandt, N.D.; Teplyakov, A.; Tomasevic, N.; Bernstein, H.D.
    Evidence for a novel GTPase priming step in the SRP protein targeting pathway (2001), EMBO J., 20, 6724-6734.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.5.4

Activating Compound

Activating Compound Comment Organism Structure
FtsY GTPase subunit of the SRP receptor activates Ffh, Ffh and FtsY reciprocally regulate each other’s GTPase activity Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
A192D reduced GTP hydrolysis, no effect on the interaction with FtsY Escherichia coli
A254L no growth defect Escherichia coli
D253N no growth defect Escherichia coli
G110S reduced GTP hydrolysis, no effect on the interaction with FtsY Escherichia coli
G256A no growth defect Escherichia coli
G257A residue resides at the N-GTPase domain interface, mutation produces a lethal phenotype, it does not significantly affect Ffh function, but severely reduces interaction with FtsY Escherichia coli
L195P reduced GTP hydrolysis, no effect on the interaction with FtsY Escherichia coli
P142L reduced GTP hydrolysis, no effect on the interaction with FtsY Escherichia coli
R255N no growth defect Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 GTP pH 7.5, 25°C, wild-type SRP Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Escherichia coli protein targeting by the SRP pathway, Ffh and FtsY, the GTPase subunit of the SRP receptor, reciprocally regulate each other’s GTPase activity, they interact only in a primed conformation which requires interdomain communication GDP + phosphate
-
 ?
GTP + H2O Escherichia coli WAM121 protein targeting by the SRP pathway, Ffh and FtsY, the GTPase subunit of the SRP receptor, reciprocally regulate each other’s GTPase activity, they interact only in a primed conformation which requires interdomain communication GDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 WAM121
-
Escherichia coli WAM121
-
 WAM121
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Escherichia coli GDP + phosphate
-
 ?
GTP + H2O protein targeting by the SRP pathway, Ffh and FtsY, the GTPase subunit of the SRP receptor, reciprocally regulate each other’s GTPase activity, they interact only in a primed conformation which requires interdomain communication Escherichia coli GDP + phosphate
-
 ?
GTP + H2O
-
 Escherichia coli WAM121 GDP + phosphate
-
 ?
GTP + H2O protein targeting by the SRP pathway, Ffh and FtsY, the GTPase subunit of the SRP receptor, reciprocally regulate each other’s GTPase activity, they interact only in a primed conformation which requires interdomain communication Escherichia coli WAM121 GDP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Ffh
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli