Literature summary for 3.6.5.3 extracted from

Lin, J.; Gagnon, M.G.; Bulkley, D.; Steitz, T.A.
Conformational changes of elongation factor G on the ribosome during tRNA translocation (2015), Cell, 160, 219-227 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene fusA, recombinant expression of the N-terminal domain (74 residues) of Thermus thermophilus ribosomal protein L9 fused to the N-terminus of EF-G connecting residues 1-74 of ribosomal protein L9 and residues 8-691 of EF-G in Escherichia coli strain BL21(DE3) Star	Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified ribosomal protein L9-elongation factor G fusion protein in complex with GDP, the EF-G-GTP complex is bound on a pre-translocation ribosome (PRE ribosome) that is deficient in tRNA translocation upon EF-G binding, sitting drop vapour diffusion method, mixing of 0.1 mM protein in 10 mM Tris-HCl, pH 7.5, 200 mM KCl, 10 mM Mg(CH3COO)2, and 1 mM 2-mercaptoethanol, with X-ray diffraction structure determination and analysis at 2.0 A resolution. Only in the presence of the GDP nucleotide, and not GTP, does EF-G crystalize with the non-rotated ribosome under the experimental conditions	Thermus thermophilus

Crystallization (Comment)

Organism

purified ribosomal protein L9-elongation factor G fusion protein in complex with GDP, the EF-G-GTP complex is bound on a pre-translocation ribosome (PRE ribosome) that is deficient in tRNA translocation upon EF-G binding, sitting drop vapour diffusion method, mixing of 0.1 mM protein in 10 mM Tris-HCl, pH 7.5, 200 mM KCl, 10 mM Mg(CH3COO)2, and 1 mM 2-mercaptoethanol, with X-ray diffraction structure determination and analysis at 2.0 A resolution. Only in the presence of the GDP nucleotide, and not GTP, does EF-G crystalize with the non-rotated ribosome under the experimental conditions

Thermus thermophilus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
ribosome	-	Thermus thermophilus	5840	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP + H2O	Thermus thermophilus	-	GDP + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	P13551	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) Star by hydrophobic interaction, anion exchange chromatography, gel filtration	Thermus thermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	-	Thermus thermophilus	GDP + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
EF-G	-	Thermus thermophilus
elongation factor G	-	Thermus thermophilus
fusA	-	Thermus thermophilus
GTPase	-	Thermus thermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Thermus thermophilus

General Information

General Information	Comment	Organism
additional information	EF-G binds with GTP or GDP on the PRE ribosome. But only in the presence of the GDP nucleotide, and not GTP, does EF-G crystalize with the non-rotated ribosome under the experimental conditions	Thermus thermophilus
physiological function	EF-G-mediated tRNA translocation, mechanism, overview	Thermus thermophilus