Protein Variants | Comment | Organism |
---|---|---|
H81A | site-directed mutagenesis, impaired enzyme mutant | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Escherichia coli | - |
GDP + phosphate | - |
? | |
GTP + H2O | Escherichia coli BW25113 | - |
GDP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene lepA | - |
Escherichia coli BW25113 | - |
gene lepA | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | growth rates of wild-type and mutant strains, overview | Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Escherichia coli | GDP + phosphate | - |
? | |
GTP + H2O | - |
Escherichia coli BW25113 | GDP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LepA | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
evolution | LepA is a paralogue of elongation factor G found in all bacteria | Escherichia coli |
malfunction | in strains DEltadksA, DELTAmolR1, DELTArsgA, DELTAtatB, DELTAtonB, DELTAtolR, DELTAubiF, DELTAubiG or DELTAubiH the deletion of lepA confers a synthetic growth phenotype. The strains are compromised for gene regulation, ribosome assembly, transport and/or respiration. Loss of LepA alters the average ribosome density for hundreds of mRNA coding regions in the cell, substantially reducing average ribosome density in many cases, but only subtle and codon-specific changes in ribosome distribution along mRNA are seen. Global perturbation of gene expression in the DELTAlepA mutant likely explains most of its phenotypes. LepA variants lacking the active-site histidine or unique C-terminal domain fail to complement the synthetic phenotypes | Escherichia coli |
additional information | histidine 81 is critical for GTPase activity, both the C-terminal domain and the GTPase activity of LepA are critical for its function in vivo | Escherichia coli |
physiological function | LepA contributes mainly to the initiation phase of translation. The effect of LepA on average ribosome density is related to the sequence of the Shine-Dalgarno region. But the enzyme does not generally influence polypeptide chain elongation rate | Escherichia coli |