Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.5.3 extracted from

  • Balakrishnan, R.; Oman, K.; Shoji, S.; Bundschuh, R.; Fredrick, K.
    The conserved GTPase LepA contributes mainly to translation initiation in Escherichia coli (2014), Nucleic Acids Res., 42, 13370-13383.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.5.3

Protein Variants

Protein Variants Comment Organism
H81A site-directed mutagenesis, impaired enzyme mutant Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Escherichia coli
-
 GDP + phosphate
-
 ?
GTP + H2O Escherichia coli BW25113
-
 GDP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 gene lepA
-
Escherichia coli BW25113
-
 gene lepA
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information growth rates of wild-type and mutant strains, overview Escherichia coli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Escherichia coli GDP + phosphate
-
 ?
GTP + H2O
-
 Escherichia coli BW25113 GDP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
LepA
-
 Escherichia coli

General Information

General Information Comment Organism
evolution LepA is a paralogue of elongation factor G found in all bacteria Escherichia coli
malfunction in strains DEltadksA, DELTAmolR1, DELTArsgA, DELTAtatB, DELTAtonB, DELTAtolR, DELTAubiF, DELTAubiG or DELTAubiH the deletion of lepA confers a synthetic growth phenotype. The strains are compromised for gene regulation, ribosome assembly, transport and/or respiration. Loss of LepA alters the average ribosome density for hundreds of mRNA coding regions in the cell, substantially reducing average ribosome density in many cases, but only subtle and codon-specific changes in ribosome distribution along mRNA are seen. Global perturbation of gene expression in the DELTAlepA mutant likely explains most of its phenotypes. LepA variants lacking the active-site histidine or unique C-terminal domain fail to complement the synthetic phenotypes Escherichia coli
additional information histidine 81 is critical for GTPase activity, both the C-terminal domain and the GTPase activity of LepA are critical for its function in vivo Escherichia coli
physiological function LepA contributes mainly to the initiation phase of translation. The effect of LepA on average ribosome density is related to the sequence of the Shine-Dalgarno region. But the enzyme does not generally influence polypeptide chain elongation rate Escherichia coli