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Literature summary for 3.6.5.3 extracted from

  • Huang, B.; Wu, H.; Hao, N.; Blombach, F.; van der Oost, J.; Li, X.; Zhang, X.C.; Rao, Z.
    Functional study on GTP hydrolysis by the GTP-binding protein from Sulfolobus solfataricus, a member of the HflX family (2010), J. Biochem., 148, 103-113.
    View publication on PubMed
Search for more literature for 3.6.5.3

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of wild-type enzyme/GDP comple and mutant enzymes G235P and G235S are grown using hanging drop method at 16°C Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
F236P kcat/Km is 63% compared to wild-type value Saccharolobus solfataricus
G235P complete loss of GTP hydrolyzing activity Saccharolobus solfataricus
G235S partial loss of GTP hydrolyzing activity Saccharolobus solfataricus
N189P complete loss of GTP hydrolyzing activity Saccharolobus solfataricus
T193N complete loss of GTP hydrolyzing activity Saccharolobus solfataricus
T213V kcat is 45% compared to the wild-type value Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0053
-
 GTP pH 7.5, 50°C, wild-type enzyme Saccharolobus solfataricus
0.011
-
 GTP pH 7.5, 50°C, mutant enzyme F236P Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requires Mg2+ for its full GTPase catalytic activity Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q980M3
-
-
Saccharolobus solfataricus P2 Q980M3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Saccharolobus solfataricus GDP + phosphate
-
 ?
GTP + H2O
-
 Saccharolobus solfataricus P2 GDP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
SsGBP
-
 Saccharolobus solfataricus
SSO0269 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0004
-
 GTP pH 7.5, 50°C, wild-type enzyme Saccharolobus solfataricus
0.00053
-
 GTP pH 7.5, 50°C, mutant enzyme F236P Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.048
-
 GTP pH 7.5, 50°C, mutant enzyme F236P Saccharolobus solfataricus
0.076
-
 GTP pH 7.5, 50°C, wild-type enzyme Saccharolobus solfataricus