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Literature summary for 3.6.5.3 extracted from

  • Walter, J.D.; Hunter, M.; Cobb, M.; Traeger, G.; Spiegel, P.C.
    Thiostrepton inhibits stable 70S ribosome binding and ribosome-dependent GTPase activation of elongation factor G and elongation factor 4 (2012), Nucleic Acids Res., 40, 360-370.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.5.3

Inhibitors

Inhibitors Comment Organism Structure
Thiostrepton inhibits the ribosome-stimulated GTPase activity of EF-G and EF4. An EF-G mutant lacking domains 4 and 5 is insensitive to the effects of thiostrepton on both GTPase activity and ribosome binding Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Escherichia coli
-
 GDP + phosphate
-
 ?
GTP + H2O Escherichia coli MRE 600
-
 GDP + phosphate
-
 ?
additional information Escherichia coli EF4-ribosome interactions during reverse translocation, overview ?
-
 ?
additional information Escherichia coli MRE 600 EF4-ribosome interactions during reverse translocation, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli MRE 600
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Escherichia coli GDP + phosphate
-
 ?
GTP + H2O reaction using Escherichia coli 70S ribosomes, determination of binding of GTPases to 70S ribosomes in the GTP state, formation of 70S ribosome-tRNAPhe -GTPase-GDPNP complexes, multiple-turnover GTP hydrolysis Escherichia coli GDP + phosphate
-
 ?
GTP + H2O
-
 Escherichia coli MRE 600 GDP + phosphate
-
 ?
GTP + H2O reaction using Escherichia coli 70S ribosomes, determination of binding of GTPases to 70S ribosomes in the GTP state, formation of 70S ribosome-tRNAPhe -GTPase-GDPNP complexes, multiple-turnover GTP hydrolysis Escherichia coli MRE 600 GDP + phosphate
-
 ?
additional information EF4-ribosome interactions during reverse translocation, overview Escherichia coli ?
-
 ?
additional information EF4-ribosome interactions during reverse translocation, overview Escherichia coli MRE 600 ?
-
 ?

Synonyms

Synonyms Comment Organism
EF-G
-
 Escherichia coli
EF4
-
 Escherichia coli
elongation factor 4
-
 Escherichia coli
elongation factor G
-
 Escherichia coli
LepA
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli

General Information

General Information Comment Organism
malfunction an EF-G mutant lacking domains 4 and 5 shows ribosome-stimulated GTP hydrolysis activity 2.5fold slower than that of wild-type full-length EF-G and is insensitive to the effects of thiostrepton on both GTPase activity and ribosome binding Escherichia coli
physiological function EF-G and EF4 perform ribosome-dependent GTP hydrolysis and bind to conserved bases in 23S rRNA and stabilize ribosomal ratcheting Escherichia coli