Literature summary for 3.6.5.3 extracted from

Ruggiero, I.; Cantiello, P.; Lamberti, A.; Sorrentino, A.; Martucci, N.M.; Ruggiero, A.; Arcone, R.; Vitagliano, L.; Arcari, P.; Masullo, M.
Biochemical characterisation of the D60A mutant of the elongation factor 1alpha from the archaeon Sulfolobus solfataricus (2009), Biochimie, 91, 835-842.

Search for more literature for 3.6.5.3

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21	Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Saccharolobus solfataricus

Protein Variants

Protein Variants	Comment	Organism
D60A	1.4fold slower hydrolysis of GTP	Saccharolobus solfataricus

Inhibitors

Inhibitors	Comment	Organism	Structure
GDP	-	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0018	-	GTP	mutant D60A	Saccharolobus solfataricus
0.0027	-	GTP	-	Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
49000	-	gel filtration	Saccharolobus solfataricus
49000	-	SDS-PAGE	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	60°C	Saccharolobus solfataricus	GDP + phosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	1*49000, SDS-PAGE and gel filtration	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
EF 1 alpha	-	Saccharolobus solfataricus
elongation factor 1 alpha	-	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	assay at	Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
87	96	half-inactivation time 99 min at 87°C, half-inactivation time 3.5 min at 96°C	Saccharolobus solfataricus
93.6	-	temperature for half denaturation of D60A mutant	Saccharolobus solfataricus
95.1	-	temperature for half denaturation	Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0008	-	GDP	-	Saccharolobus solfataricus
0.0029	-	GDP	mutant D60A	Saccharolobus solfataricus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)