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Literature summary for 3.6.5.3 extracted from

  • Raimo, G.; Masullo, M.; Bocchini, V.
    Studies on the polypeptide elongation factor 2 from Sulfolobus solfataricus. Interaction with guanosine nucleotides and GTPase activity stimulated by ribosomes (1995), J. Biol. Chem., 270, 21082-21085.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
GDP competitive with GTP Saccharolobus solfataricus
guanyl-5'-yl imidodiphosphate competitive with GTP Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
GTP pH 7.8, 50°C Saccharolobus solfataricus
0.009
-
GTP pH 7.8, 60°C Saccharolobus solfataricus
0.01
-
GTP pH 7.8, 70°C Saccharolobus solfataricus
0.011
-
GTP pH 7.8, 75°C Saccharolobus solfataricus
0.013
-
GTP pH 7.8, 80°C Saccharolobus solfataricus
0.032
-
GTP pH 7.8, 87°C Saccharolobus solfataricus
0.148
-
GTP pH 7.8, 91°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
elongation factor 2 exhibits an intrinsic hardly detectable GTPase activity that is stimulated by ribosomes up to 2000-fold
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
Saccharolobus solfataricus GDP + phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
both Km for GTP and kcat of GTPaser increase with increasing temperature Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
GTP pH 7.8, 50°C Saccharolobus solfataricus
0.8
-
GTP pH 7.8, 60°C Saccharolobus solfataricus
1.7
-
GTP pH 7.8, 70°C Saccharolobus solfataricus
2.7
-
GTP pH 7.8, 75°C Saccharolobus solfataricus
3.5
-
GTP pH 7.8, 80°C Saccharolobus solfataricus
6.2
-
GTP pH 7.8, 87°C Saccharolobus solfataricus
8.8
-
GTP pH 7.8, 91°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Saccharolobus solfataricus