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Literature summary for 3.6.5.3 extracted from
- Archaeal elongation factor 1alpha from Sulfolobus solfataricus interacts with the eubacterial antibiotic GE2270A (2004), Extremophiles, 8, 499-505.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | - |
Saccharolobus solfataricus |  |
| ribosome | - |
Saccharolobus solfataricus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GE2270A | antibiotic inhibits intrinsic GTPase and that stimulated by ribosomes; thiazolyl-peptide antibiotic, inhibits both the intrinsic GTPase of elongation factor 1alpha and that stimulated by ribosomes. The M domain is the region of the enzyme most responsible for the interaction with GE2270A | Saccharolobus solfataricus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | Saccharolobus solfataricus | - |
GDP + phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
| Saccharolobus solfataricus | - |
elongatipon factor 1alpha | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | - |
Saccharolobus solfataricus | GDP + phosphate | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EF-1alpha | - |
Saccharolobus solfataricus |
| elongation factor 1alpha | - |
Saccharolobus solfataricus |
| protein-sythesizing GTPase | - |
Saccharolobus solfataricus |
| SsEF-1alpha | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
the GTPase activity is measured in the presence of either 3.6 M NaCl or 1.6 microM ribosomes | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Saccharolobus solfataricus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0059 | - |
half-inhibition of the GTPase of the isolated domain GM of SsEF-1alpha | Saccharolobus solfataricus | GE2270A | |
| 0.007 | - |
half-inhibition of the NaCl-dependent GTPase of SsEF-1alpha | Saccharolobus solfataricus | GE2270A | |
| 0.011 | - |
half-inhibition of the ribosome-dependent GTPase of SsEF-1alpha | Saccharolobus solfataricus | GE2270A | |
| 0.0148 | - |
half-inhibition of the GTPase of the isolated domain G of SsEF-1alpha | Saccharolobus solfataricus | GE2270A | |
| 0.0463 | - |
half-inhibition of the GTPase of a chimeric EF containing the domain G of SsEF-1alpha and the domains MC of Escherichia coli EF-Tu | Saccharolobus solfataricus | GE2270A | |
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