Cloned (Comment) | Organism |
---|---|
recombinant SsEF-1alpha and its nucleotide-free form are prepared by using an Escherichia coli expression system | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guanidine hydrochloride | deactivation by denaturation of the protein | Saccharolobus solfataricus | |
Urea | deactivation by denaturation of the protein | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Saccharolobus solfataricus | - |
GDP + phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus | - |
elongation factor 1alpha | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Renatured (Comment) | Organism |
---|---|
denaturation by urea and guanidine hydrochloride shows a cooperative unfolding process with no intermediate species. Chemical unfolding by urea and guanidine hydrochloride is fully reversible for both enzyme-GDP complex and nucleotide-free enzyme. Both forms exhibit remarkable stability against urea, but not against guanidine hydrochloride | Saccharolobus solfataricus |
Storage Stability | Organism |
---|---|
-20°C, 20 mM Tris/HCl, pH 7.8, 50 mM KCl, 10 mM MgCl2, 50% v/v glycerol | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Saccharolobus solfataricus | GDP + phosphate | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
elongation factor 1alpha | - |
Saccharolobus solfataricus |
protein-synthesizing GTPase | - |
Saccharolobus solfataricus |
SsEF-1alpha | - |
Saccharolobus solfataricus |