Literature summary for 3.6.5.3 extracted from

Granata, V.; Graziano, G.; Ruggiero, A.; Raimo, G.; Masullo, M.; Arcari, P.; Vitagliano, L.; Zagari, A.
Chemical denaturation of the elongation factor 1alpha isolated from the hyperthermophilic archaeon Sulfolobus solfataricus (2006), Biochemistry, 45, 719-726.

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Cloned(Commentary)

Cloned (Comment)	Organism
recombinant SsEF-1alpha and its nucleotide-free form are prepared by using an Escherichia coli expression system	Saccharolobus solfataricus

Inhibitors

Inhibitors	Comment	Organism	Structure
guanidine hydrochloride	deactivation by denaturation of the protein	Saccharolobus solfataricus
Urea	deactivation by denaturation of the protein	Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP + H2O	Saccharolobus solfataricus	-	GDP + phosphate	-	ir

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-
Saccharolobus solfataricus	-	elongation factor 1alpha	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus solfataricus

Renatured (Commentary)

Renatured (Comment)	Organism
denaturation by urea and guanidine hydrochloride shows a cooperative unfolding process with no intermediate species. Chemical unfolding by urea and guanidine hydrochloride is fully reversible for both enzyme-GDP complex and nucleotide-free enzyme. Both forms exhibit remarkable stability against urea, but not against guanidine hydrochloride	Saccharolobus solfataricus

Storage Stability

Storage Stability	Organism
-20°C, 20 mM Tris/HCl, pH 7.8, 50 mM KCl, 10 mM MgCl2, 50% v/v glycerol	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	-	Saccharolobus solfataricus	GDP + phosphate	-	ir

Synonyms

Synonyms	Comment	Organism
elongation factor 1alpha	-	Saccharolobus solfataricus
protein-synthesizing GTPase	-	Saccharolobus solfataricus
SsEF-1alpha	-	Saccharolobus solfataricus

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Release 2023.1 (January 2023)