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Literature summary for 3.6.5.2 extracted from

  • Wong, H.; Akamatsu, A.; Wang, Q.; Higuchi, M.; Matsuda, T.; Okuda, J.; Kosami, K.; Inada, N.; Kawasaki, T.; Kaneko-Kawano, T.; Nagawa, S.; Tan, L.; Kawano, Y.; Shimamoto, K.
    In vivo monitoring of plant small GTPase activation using a Foerster resonance energy transfer biosensor (2018), Plant Methods, 14, 56 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of Raichu-OsRac1 in rice protoplasts from strain C5924 with high efficiency Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Oryza sativa Japonica Group
-
GDP + phosphate
-
?
GTP + H2O Oryza sativa Japonica Group C5924
-
GDP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group Q9SSX0
-
-
Oryza sativa Japonica Group C5924 Q9SSX0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
Oryza sativa Japonica Group GDP + phosphate
-
?
GTP + H2O
-
Oryza sativa Japonica Group C5924 GDP + phosphate
-
?

Synonyms

Synonyms Comment Organism
OsRac1
-
Oryza sativa Japonica Group
Rac-like GTP-binding protein 1 UniProt Oryza sativa Japonica Group
small GTPase
-
Oryza sativa Japonica Group

General Information

General Information Comment Organism
evolution the enzyme belongs to the Rac/ROP family small GTPases Oryza sativa Japonica Group
additional information establishment of a plant version of a Förster resonance energy transfer (FRET) probe called Ras and interacting protein chimeric unit (Raichu) that can successfully monitor activation of the rice small GTPase OsRac1 during various defence responses in cells, protocol for visualizing spatiotemporal activity of plant Rac/ ROP GTPase in living plant cells, transfection of rice protoplasts, method, detailed overview Oryza sativa Japonica Group
physiological function small GTPases act as molecular switches that regulate various plant responses such as disease resistance, pollen tube growth, root hair development, cell wall patterning and hormone responses. Small GTPases exhibit both GDP/GTP-binding activity and GTPase activity, and work as molecular switches by cycling between GDP-bound inactive and GTP-bound active forms. Rac/ROP family small GTPases are master regulators, controlling various signalling systems in plants such as those governing pollen tube growth, root hair development, auxin signaling, and disease resistance Oryza sativa Japonica Group