Activating Compound | Comment | Organism | Structure |
---|---|---|---|
TGF-beta1 | TGF-beta1 activates Rap1 through cAMP and Epac1/Epac2. 2',5-dideoxyadenosine is an inhibitor of adenlyate cyclase on Rap1 activity | Mus musculus |
Cloned (Comment) | Organism |
---|---|
recombinant expression of GST-tagged RapI protein | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Mus musculus | - |
GDP + phosphate | - |
? | |
GTP + H2O | Mus musculus C57BL/6 | - |
GDP + phosphate | - |
? | |
additional information | Mus musculus | Ras-related, small GTPases act as molecular switches that control a variety of cellular processes by cycling between alternative conformational states: in the active state, they are bound with GTP, and in the inactive state, they are bound with GDP. In their active state, GTPases recognize their target effector proteins and evoke responses until GTP hydrolysis returns the switch to the off position | ? | - |
? | |
additional information | Mus musculus C57BL/6 | Ras-related, small GTPases act as molecular switches that control a variety of cellular processes by cycling between alternative conformational states: in the active state, they are bound with GTP, and in the inactive state, they are bound with GDP. In their active state, GTPases recognize their target effector proteins and evoke responses until GTP hydrolysis returns the switch to the off position | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q91VL8 | - |
- |
Mus musculus | Q9QUI0 | - |
- |
Mus musculus C57BL/6 | Q91VL8 | - |
- |
Mus musculus C57BL/6 | Q9QUI0 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
macrophage | - |
Mus musculus | - |
RAW-264.7 cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Mus musculus | GDP + phosphate | - |
? | |
GTP + H2O | - |
Mus musculus C57BL/6 | GDP + phosphate | - |
? | |
additional information | Ras-related, small GTPases act as molecular switches that control a variety of cellular processes by cycling between alternative conformational states: in the active state, they are bound with GTP, and in the inactive state, they are bound with GDP. In their active state, GTPases recognize their target effector proteins and evoke responses until GTP hydrolysis returns the switch to the off position | Mus musculus | ? | - |
? | |
additional information | Ras-related, small GTPases act as molecular switches that control a variety of cellular processes by cycling between alternative conformational states: in the active state, they are bound with GTP, and in the inactive state, they are bound with GDP. In their active state, GTPases recognize their target effector proteins and evoke responses until GTP hydrolysis returns the switch to the off position | Mus musculus C57BL/6 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Rap1 | - |
Mus musculus |
RhoA | - |
Mus musculus |
small GTPase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |
Organism | Comment | Expression |
---|---|---|
Mus musculus | 8CPT-2Me-cAMP suppresses GTP-RhoA levels | down |
Mus musculus | si-RNAs of Epac1 and Epac2 reduced GTP-Rap1 levels | down |
Mus musculus | si-Rap1 augments GTP-RhoA levels and cell migration. si-RNAs of Epac1 and Epac2 reduced GTP-Rap1 levels leading to promotion of GTP-RhoA levels. si-RNA of ARAP3 (Rap-dependent RhoGAP) increased GTP-RhoA level and cell migration | up |
General Information | Comment | Organism |
---|---|---|
physiological function | small GTPase Rap1 regulates cell migration through regulation of small GTPase RhoA activity in response to transforming growth factor-beta1. GTP-RhoA and GTP-Rap1 levels are reciprocally regulated in a time-dependent manner following TGF-beta1 stimulation. Ras-related, small GTPases act as molecular switches that control a variety of cellular processes by cycling between alternative conformational states. In the active state, they are bound with GTP, and in the inactive state, they are bound with GDP. In their active state, GTPases recognize their target effector proteins and evoke responses until GTP hydrolysis returns the switch to the off position. GTP-binding to small GTPases is catalyzed by guanine nucleotide exchange factors (GEFs), and hydrolysis of the bound GTP is accelerated by GTPase activating proteins (GAPs), Downstream effector proteins of Rap1, including RapL, AF6, and ARAP3 transmit the activated Rap1 signals within the cells. Rap1 regulates GTPase RhoA activity, but RhoA does not regulate the Rap1 activity | Mus musculus |
physiological function | small GTPase Rap1 regulates cell migration through regulation of small GTPase RhoA activity in response to transforming growth factor-beta1. GTP-RhoA and GTP-Rap1 levels are reciprocally regulated in a time-dependent manner following TGF-beta1 stimulation. Ras-related, small GTPases act as molecular switches that control a variety of cellular processes by cycling between alternative conformational states. In the active state, they are bound with GTP, and in the inactive state, they are bound with GDP. In their active state, GTPases recognize their target effector proteins and evoke responses until GTP hydrolysis returns the switch to the off position. GTP-binding to small GTPases is catalyzed by guanine nucleotide exchange factors (GEFs), and hydrolysis of the bound GTP is accelerated by GTPase activating proteins (GAPs). Downstream effector proteins of Rap1, including RapL, AF6, and ARAP3 transmit the activated Rap1 signals within the cells. Rap1 regulates GTPase RhoA activity, but RhoA does not regulate the Rap1 activity | Mus musculus |