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Literature summary for 3.6.5.2 extracted from
- Purification, crystallization and preliminary X-ray crystallographic analysis of Rab27a GTPase in complex with exophilin4/Slp2-a effector (2008), Acta Crystallogr. Sect. F, 64, 599-601.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain BL21(DE3)-LysS | Mus musculus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapour diffusion method, using 1.0 M sodium citrate trihydrate, 0.1 M sodium cacodylate, at pH 6.5 | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E78L | inactive | Mus musculus |
| E78L/C123S/C188S | the mutant may modulate effector recognition by forming an intramolecular disulfide bridge | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| GppNHp-bound Rab27a in complex with the Rab27-binding domain of exophilin4/Slp2-a effector, glutathione Sepharose 4B resin chromatography and Superdex-75 gel filtration | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Rab27a GTPase | - |
Mus musculus |
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