Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pisum sativum |
expression in Escherichia coli | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
sitting-drop vapor-diffusion technique, structure of the GMPPNP-bound state and GMP-bound state of Toc33 GTPase | Arabidopsis thaliana |
sitting-drop vapor-diffusion technique, structure of the GMPPNP-bound state of Toc33/34 GTPase | Pisum sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Pisum sativum | transport of precursor proteins across chloroplast membranes involves the GTPases Toc33/34 and Toc159 at the outer chloroplast envelope | GDP + phosphate | - |
? | |
GTP + H2O | Arabidopsis thaliana | transport of precursor proteins across chloroplast membranes involves the GTPases Toc33/34 and Toc159 at the outer chloroplast envelope | GDP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O23680 | - |
- |
Pisum sativum | Q41009 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
the cytosolic G domain of psToc34 (lacking the C-terminal membrane anchor) is purified mainly in the GDP-bound form after recombinant protein production in Escherichia coli | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | transport of precursor proteins across chloroplast membranes involves the GTPases Toc33/34 and Toc159 at the outer chloroplast envelope | Pisum sativum | GDP + phosphate | - |
? | |
GTP + H2O | transport of precursor proteins across chloroplast membranes involves the GTPases Toc33/34 and Toc159 at the outer chloroplast envelope | Arabidopsis thaliana | GDP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | the small GTPase Toc33/34 can homodimerize. Dimerization is independent of nucleotide loading state. GTPase activation by dimerization is sparse. The dimer is not self-activating, possibly lacking an additional regulatory layer | Pisum sativum |
monomer | - |
Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
GTPase Toc33 | - |
Arabidopsis thaliana |
GTPase Toc33/34 | - |
Pisum sativum |