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Literature summary for 3.6.5.2 extracted from

  • Koenig, P.; Oreb, M.; Hoefle, A.; Kaltofen, S.; Rippe, K.; Sinning, I.; Schleiff, E.; Tews, I.
    The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures (2008), Structure, 16, 585-596.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pisum sativum
expression in Escherichia coli Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapor-diffusion technique, structure of the GMPPNP-bound state and GMP-bound state of Toc33 GTPase Arabidopsis thaliana
sitting-drop vapor-diffusion technique, structure of the GMPPNP-bound state of Toc33/34 GTPase Pisum sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Pisum sativum transport of precursor proteins across chloroplast membranes involves the GTPases Toc33/34 and Toc159 at the outer chloroplast envelope GDP + phosphate
-
?
GTP + H2O Arabidopsis thaliana transport of precursor proteins across chloroplast membranes involves the GTPases Toc33/34 and Toc159 at the outer chloroplast envelope GDP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O23680
-
-
Pisum sativum Q41009
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arabidopsis thaliana
the cytosolic G domain of psToc34 (lacking the C-terminal membrane anchor) is purified mainly in the GDP-bound form after recombinant protein production in Escherichia coli Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O transport of precursor proteins across chloroplast membranes involves the GTPases Toc33/34 and Toc159 at the outer chloroplast envelope Pisum sativum GDP + phosphate
-
?
GTP + H2O transport of precursor proteins across chloroplast membranes involves the GTPases Toc33/34 and Toc159 at the outer chloroplast envelope Arabidopsis thaliana GDP + phosphate
-
?

Subunits

Subunits Comment Organism
dimer the small GTPase Toc33/34 can homodimerize. Dimerization is independent of nucleotide loading state. GTPase activation by dimerization is sparse. The dimer is not self-activating, possibly lacking an additional regulatory layer Pisum sativum
monomer
-
Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
GTPase Toc33
-
Arabidopsis thaliana
GTPase Toc33/34
-
Pisum sativum