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Literature summary for 3.6.5.2 extracted from

  • Chopade, B.A.; Shankar, S.; Sundin, G.W.; Mukhopadhyay, S.; Chakrabarty, A.M.
    Characterization of membrane-associated Pseudomonas aeruginosa Ras-like protein Pra, a GTP-binding protein that forms complexes with truncated nucleoside diphosphate kinase and pyruvate kinase to modulate GTP synthesis (1997), J. Bacteriol., 179, 2181-2188.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
nucleoside diphosphate kinase truncated 12-kDa form, builds complex with Pra with increased GTP and dGTP synthesis activity and decreased CTP and UTP or dCTP and dUTP synthesis activity relative to their synthesis by uncomplexed Ndk Pseudomonas aeruginosa
pyruvate kinase builds complex with Pra with specific GTP synthesis activity Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Pseudomonas aeruginosa 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Pseudomonas aeruginosa
-
GDP + phosphate
-
?
GTP + H2O Pseudomonas aeruginosa 8830
-
GDP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa 8830
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000283
-
substrate GTP, pH 7.6, 25°C, 30-60 min Pseudomonas aeruginosa

Storage Stability

Storage Stability Organism
-70°C, 50 mM Tris-HCl, 10 mM MgCl2, 1 mM dithiothreitol, 0.5% glycerol, pH 7.6, 50% glycerol Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
Pseudomonas aeruginosa GDP + phosphate
-
?
GTP + H2O
-
Pseudomonas aeruginosa 8830 GDP + phosphate
-
?

Synonyms

Synonyms Comment Organism
PRA
-
Pseudomonas aeruginosa