Activating Compound | Comment | Organism | Structure |
---|---|---|---|
nucleoside diphosphate kinase | truncated 12-kDa form, builds complex with Pra with increased GTP and dGTP synthesis activity and decreased CTP and UTP or dCTP and dUTP synthesis activity relative to their synthesis by uncomplexed Ndk | Pseudomonas aeruginosa | |
pyruvate kinase | builds complex with Pra with specific GTP synthesis activity | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Pseudomonas aeruginosa | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | Pseudomonas aeruginosa | - |
GDP + phosphate | - |
? | |
GTP + H2O | Pseudomonas aeruginosa 8830 | - |
GDP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa 8830 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000283 | - |
substrate GTP, pH 7.6, 25°C, 30-60 min | Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
-70°C, 50 mM Tris-HCl, 10 mM MgCl2, 1 mM dithiothreitol, 0.5% glycerol, pH 7.6, 50% glycerol | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + H2O | - |
Pseudomonas aeruginosa | GDP + phosphate | - |
? | |
GTP + H2O | - |
Pseudomonas aeruginosa 8830 | GDP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PRA | - |
Pseudomonas aeruginosa |