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Literature summary for 3.6.5.1 extracted from

  • Digby, G.J.; Lober, R.M.; Sethi, P.R.; Lambert, N.A.
    Some G protein heterotrimers physically dissociate in living cells (2006), Proc. Natl. Acad. Sci. USA, 21, 17789-17794.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
into pcDNA3.1 vector for transfection of HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
CFP-TM-Galpha consists of a cleavable signal peptide from human growth hormone, enhanced CFP, the amino-terminal amino acids of the rat mu-opioid receptor, including TM1 and intracellular loop1, and the human Galpha subunit Homo sapiens
CFP-TM-Galphai3 C to G mutation at the -4 position to render them insensitive to PTX-mediated ADP-ribosylation Homo sapiens
CFP-TM-GalphaoA C to G mutation at the -4 position to render them insensitive to PTX-mediated ADP-ribosylation Homo sapiens
CFP-TM-GalphaQ205L active mutant Homo sapiens
CFP-TM-Galphasq5 the five aminoacids normally found in the carboxyl terminus of Galphas, QYELL, are replaced with those normally found in the carboxy terminus of Galphaq, EYNLV Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
Homo sapiens 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Homo sapiens
-
GDP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
Homo sapiens GDP + phosphate
-
?

Subunits

Subunits Comment Organism
heterotrimer Galpha subunit, GDP-bound inactive, GTP-bound active and betagamma dimer Homo sapiens

Synonyms

Synonyms Comment Organism
GTP phosphohydrolase
-
Homo sapiens
heterotrimeric G-protein GTPase
-
Homo sapiens