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Literature summary for 3.6.4.B1 extracted from

  • Umeki, N.; Mitsui, T.; Umezu, N.; Kondo, K.; Maruta, S.
    Preparation and characterization of a novel rice plant-specific kinesin (2006), J. Biochem., 139, 645-654.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
microtubule up to 10fold activation. Half-maximal concentration for activation 0.005 mM Oryza sativa

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli of full-length protein and motor domain alone Oryza sativa
expression of the motor domain of kinesin K16 in Escherichia coli Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
NaCl basal activity is independent of NaCl, microtubule-activated enzyme shows 50% inhibition at around 40 mM Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
microtubule in the absence of nucleotides, 75% of K16 is associated with microtubules. In presence of ADP and ATP, 55% and 65%, respectively, are associated Oryza sativa 5874
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
x * 60000, calculated for full-length protein, x * 38000, SDS-PAGE and calculated for motor domain Oryza sativa
60000
-
x * 60000, calculated for full-length protein, x * 38000, SDS-PAGE and calculated for motor domain Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Oryza sativa
-
ADP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant kinesin motor domain Oryza sativa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.62
-
pH 7.0, 25°C Oryza sativa
16.2
-
pH 7.0, 25°C, presence of microtubules Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
Oryza sativa ADP + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 60000, calculated for full-length protein, x * 38000, SDS-PAGE and calculated for motor domain Oryza sativa

Synonyms

Synonyms Comment Organism
kinesin K16
-
Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
basal activity Oryza sativa
6
-
microtubule-activated enzyme Oryza sativa