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Literature summary for 3.6.4.7 extracted from
- ATP-dependent assembly of the heteromeric Pex1p-Pex6p-complex of the peroxisomal matrix protein import machinery (2012), J. Struct. Biol., 179, 126-132.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| organization and cloning of a recombinant Pex1p/Pex6p-complex, recombinant expression of the N-terminally His6-tagged and C-terminally GST-tagged Pex1p/His-Pex6p-complex in Escherichia coli strain Tuner(DE3), ATP-dependence of the Pex1p/Pex6p complex formation | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | disassembly of the complex into its Pex1p and Pex6p subunits is observed upon ATP-depletion; disassembly of the complex into its Pex1p and Pex6p subunits is observed upon ATP-depletion | Saccharomyces cerevisiae | |
| NEM | in the presence of N-ethylmaleimide, ATPase activity of the peroxisomal AAA-complex is drastically decreased and the complex dissociates; in the presence of N-ethylmaleimide, ATPase activity of the peroxisomal AAA-complex is drastically decreased and the complex dissociates | Saccharomyces cerevisiae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
ATP | recombinant Pex1p/Pex6p complex, pH 7.4, 37°C | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| peroxisome | - |
Saccharomyces cerevisiae | 5777 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
for Pex6p, the effect of ATP is not as striking but it is obvious that the yield for monomeric Pex6p is decreased and higher molecular weight species increased, which again might indicate aggregation | Saccharomyces cerevisiae |
| 360000 | - |
Pex1p trimeric complex in the presence of ATP, without ATP Pex1p seems to form higher molecular weight structures, most likely aggregates | Saccharomyces cerevisiae |
| 700000 | - |
recombinant Pex1p/Pex6p complex, gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Saccharomyces cerevisiae | - |
ADP + phosphate | - |
? | |
| additional information | Saccharomyces cerevisiae | The recombinant AAA-complex exhibits an ATPase activity | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P24004 | PEX1 | - |
| Saccharomyces cerevisiae | P33760 | PEX6 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His6-tagged and C-terminally GST-tagged Pex1p/His-Pex6p-complex from Escherichia coli strain Tuner(DE3) by nickel affinity chromatography and gel filtration | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Saccharomyces cerevisiae | ADP + phosphate | - |
? | |
| additional information | The recombinant AAA-complex exhibits an ATPase activity | Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterohexamer | Pex1p and Pex6p interact and form a heterohexameric complex in a one-to-one ratio of both AAA-proteins | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pex1p | - |
Saccharomyces cerevisiae |
| Pex1p-Pex6p-complex | - |
Saccharomyces cerevisiae |
| Pex6p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Saccharomyces cerevisiae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | Pex1p and Pex6p belong to the group of type-II AAA proteins characterized by the presence of two AAA-domains, termed D1 and D2, post-positioned to an N-terminal domain | Saccharomyces cerevisiae |
| additional information | Pex1p and Pex6p interact and form a heteromeric complex. Disassembly of the complex into its Pex1p and Pex6p subunits is observed upon ATP-depletion, indicating that formation of the Pex1p/Pex6p-complex requires the presence of ATP | Saccharomyces cerevisiae |
| physiological function | the peroxisomal matrix protein import is facilitated by soluble receptor molecules which cycle between cytosol and the peroxisomal membrane. At the end of the receptor cycle, the import receptors are exported back to the cytosol in an ATP-dependent manner catalyzed by a complex of Pex1p and Pex6p | Saccharomyces cerevisiae |
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