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Literature summary for 3.6.4.6 extracted from
- Nucleotide-dependent conformational changes and assembly of the AAA ATPase SKD1/VPS4B (2008), Traffic, 9, 2180-2189.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) pLysE cells | Mus musculus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapour diffusion method, using 5% (w/v) polyethylene glycol 3350 and 100 mM MES (pH 6.0) | Mus musculus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
apo-form, Guinier analysis | Mus musculus |
| 50000 | - |
ADP-bound form, Guinier analysis | Mus musculus |
| 148000 | - |
ATP-bound form, Guinier analysis | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | P46467 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, DEAE Sepharose column chromatography, and Superdex 75 gel filtration | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Mus musculus | ADP + phosphate | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | addition of ATP but not ADP increases the oligomerized population of SKD1 | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SKD1/VPS4B | - |
Mus musculus |
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Release 2023.1 (January 2023)
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