Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 (DE3) cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
K251A | the p97 mutant carrying mutations in the D2 domain shows little ATPase activity when compared with wild type enzyme | Rattus norvegicus |
K251A | the Walker A mutation to the D1 domain alone shows a moderate decrease in ATPase activity, has reduced affinity for nucleotide in the D1 domain and so contains little prebound ADP | Rattus norvegicus |
K524A | the p97 mutant carrying mutations in the D2 domain shows little ATPase activity when compared with wild type enzyme | Rattus norvegicus |
R359A | the p97 mutant carrying mutations in the D2 domain shows little ATPase activity when compared with wild type enzyme | Rattus norvegicus |
R635A | the p97 mutant carrying mutations in the D2 domain shows little ATPase activity when compared with wild type enzyme | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P46462 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Ni2+-chelating column chromatography | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Rattus norvegicus | ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | x-ray crystallography | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
peptide p97 | essential chaperone in endoplasmic reticulum-associated degradation and organelle biogenesis, contains two ATPases associated with various cellular activities domains (D1 and D2) | Rattus norvegicus |
vasolin-containing protein | - |
Rattus norvegicus |
VCP | - |
Rattus norvegicus |