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Literature summary for 3.6.4.6 extracted from
- Endosomal transport function in yeast requires a novel AAA-type ATPase, Vps4p (1997), EMBO J., 16, 1820-1831.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| VPS4 gene cloned and overexpressed in Escherichia coli XL1-blue, using a GST fusion vector | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-ethylmaleimide | - |
Saccharomyces cerevisiae |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
| endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
| endoplasmic reticulum | - |
Saccharomyces cerevisiae | 5783 | - |
| Golgi apparatus | - |
Homo sapiens | 5794 | - |
| Golgi apparatus | - |
Saccharomyces cerevisiae | 5794 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
predicted from amino acid sequence | Saccharomyces cerevisiae |
| 60000 | - |
recombinant enzyme, purified from E. coli, gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Mus musculus | - |
ADP + phosphate | - |
? | |
| ATP + H2O | Homo sapiens | - |
ADP + phosphate | - |
? | |
| ATP + H2O | Saccharomyces cerevisiae | - |
ADP + phosphate | - |
? | |
| ATP + H2O | Schizosaccharomyces pombe | - |
ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
human | - |
| Mus musculus | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Schizosaccharomyces pombe | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein, expressed in Escherichia coli XL1-blue | Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + H2O = ADP + phosphate | large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger 's syndrome. | Mus musculus | |
| ATP + H2O = ADP + phosphate | large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger 's syndrome. | Homo sapiens | |
| ATP + H2O = ADP + phosphate | large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger 's syndrome. | Saccharomyces cerevisiae | |
| ATP + H2O = ADP + phosphate | large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger 's syndrome. | Schizosaccharomyces pombe |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.45 | - |
Vps4p | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Mus musculus | ADP + phosphate | - |
? | |
| ATP + H2O | - |
Homo sapiens | ADP + phosphate | - |
? | |
| ATP + H2O | - |
Saccharomyces cerevisiae | ADP + phosphate | - |
? | |
| ATP + H2O | - |
Schizosaccharomyces pombe | ADP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Vps4p | - |
Saccharomyces cerevisiae |
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