Cloned (Comment) | Organism |
---|---|
VPS4 gene cloned and overexpressed in Escherichia coli XL1-blue, using a GST fusion vector | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
endoplasmic reticulum | - |
Saccharomyces cerevisiae | 5783 | - |
Golgi apparatus | - |
Homo sapiens | 5794 | - |
Golgi apparatus | - |
Saccharomyces cerevisiae | 5794 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
predicted from amino acid sequence | Saccharomyces cerevisiae |
60000 | - |
recombinant enzyme, purified from E. coli, gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Mus musculus | - |
ADP + phosphate | - |
? | |
ATP + H2O | Homo sapiens | - |
ADP + phosphate | - |
? | |
ATP + H2O | Saccharomyces cerevisiae | - |
ADP + phosphate | - |
? | |
ATP + H2O | Schizosaccharomyces pombe | - |
ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
human | - |
Mus musculus | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Schizosaccharomyces pombe | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein, expressed in Escherichia coli XL1-blue | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + H2O = ADP + phosphate | large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger 's syndrome. | Mus musculus | |
ATP + H2O = ADP + phosphate | large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger 's syndrome. | Homo sapiens | |
ATP + H2O = ADP + phosphate | large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger 's syndrome. | Saccharomyces cerevisiae | |
ATP + H2O = ADP + phosphate | large family of ATP-hydrolysing enzymes involved in the heterotypic fusion of membrane vesicles with target membranes and the homotypic fusion of various membrane compartments. They belong to the AAA-type (ATPase associated with a variety of cell activities) ATPase superfamily. They include peroxin, which apparently is involved in Zellweger 's syndrome. | Schizosaccharomyces pombe |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.45 | - |
Vps4p | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Mus musculus | ADP + phosphate | - |
? | |
ATP + H2O | - |
Homo sapiens | ADP + phosphate | - |
? | |
ATP + H2O | - |
Saccharomyces cerevisiae | ADP + phosphate | - |
? | |
ATP + H2O | - |
Schizosaccharomyces pombe | ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Vps4p | - |
Saccharomyces cerevisiae |