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Literature summary for 3.6.4.13 extracted from
- A cold-inducible DEAD-Box RNA helicase from Arabidopsis thaliana regulates plant growth and development under low temperature (2016), PLoS ONE, 11, e0154040 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene RH7, phylogenetic analysis, promoter:GUS transgenic plants AtRH7 quantitative real-time PCR expression analysis. AtRH7 is fused to the C-terminus of YFP and expressed in opnion cells revealing that AtRH7 localizes mainly to the nucleolus and to a minor degree to the nucleoplasm. The enzyme can complement the Escherichia coli DELTAcsdA mutant, which is deficient in growth at low temperatures | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of RH7 knockout mutant lines, several morphological alterations such as disturbed vein pattern, pointed first true leaves, and short roots, which resemble ribosome-related mutants of Arabidopsis thaliana, phenotype analysis of rh7-5 and rh7-8 mutants under 22°C, overview. Knockout mutants of AtRH7 display several morphological alterations during vegetative and reproductive growth. In addition, the mutants exhibit severe defects in germination and leaf development under long-term low temperature conditions. Accumulation of rRNA precursors in rh7 mutant plants corroborate the hypothesis that AtRH7 affects ribosome biogenesis. AtRH7 mutations affect ribosomal RNA biogenesis in the nucleolus | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | AtRH7localizes mainly to the nucleolus and to a minor degree to the nucleoplasm. AtRH7 forms a complex with AtCSP3 mainly in the nucleolus, with a smaller portion in the nucleoplasm | Arabidopsis thaliana | - |
- |
| nucleolus | - |
Arabidopsis thaliana | 5730 | - |
| nucleoplasm | - |
Arabidopsis thaliana | 5654 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Arabidopsis thaliana |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Arabidopsis thaliana | - |
ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q39189 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bud | - |
Arabidopsis thaliana | - |
| cauline leaf | - |
Arabidopsis thaliana | - |
| flower | - |
Arabidopsis thaliana | - |
| additional information | expression pattern of ATRH7 in different tissues, overview. Enzyme AtRH7 is expressed ubiquitously and its levels of the expression are higher in rapidly growing tissues, including the young rosette leaves, the lateral roots, and the root tips, while comparatively weaker activity is also detected in other parts of the plants | Arabidopsis thaliana | - |
| root | - |
Arabidopsis thaliana | - |
| rosette leaf | - |
Arabidopsis thaliana | - |
| seedling | - |
Arabidopsis thaliana | - |
| shoot | - |
Arabidopsis thaliana | - |
| silique | - |
Arabidopsis thaliana | - |
| stem | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Arabidopsis thaliana | ADP + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtRH7 | - |
Arabidopsis thaliana |
| DEAD-box RNA helicase | - |
Arabidopsis thaliana |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | DEAD-box proteins comprise the largest family of RNA helicases in plants, and exist in most organisms. They possess 12 conserved motifs that are involved in ATPase, helicase, and RNA binding activities, and participate in a variety of RNA-associated events from transcription to RNA decay. Some DEAD-box proteins are involved in the regulation of plant growth and development through ribosome biogenesis. Phylogenetic analysis, and evolutionary and functional relationships of AtRH7. AtRH7 belongs to a family whose members are involved in rRNA and mRNA processing. AtRH7 shares a conserved function with Escherichia coli enzyme CsdA under cold conditions | Arabidopsis thaliana |
| malfunction | knockout mutant lines display several morphological alterations such as disturbed vein pattern, pointed first true leaves, and short roots, which resemble ribosome-related mutants of Arabidopsis thaliana. In addition, aberrant floral development as also observed in rh7 mutants. When the mutants are germinated at low temperature (12°C), both radicle and first leaf emergence are severely delayed, after exposure of seedlings to a long period of cold, the mutants develop aberrant, fewer, and smaller leaves. RNA blots and circular RT-PCR reveal that 35S and 18S rRNA precursors accumulate to higher levels in the mutants than in wild-type under both normal and cold conditions, suggesting the mutants are partially impaired in pre-rRNA processing | Arabidopsis thaliana |
| physiological function | the cold-inducible DEAD-Box RNA helicase AtRH7 regulates plant growth and development under low temperature in Arabidopsis thaliana. AtRH7 affects rRNA biogenesis and is an interactor of Arabidopsis cold shock domain protein 3 (AtCSP3), which is an RNA chaperone involved in cold adaptation. The enzyme can complement the Escherichia coli DELTAcsdA mutant, which is deficient in growth at low temperatures | Arabidopsis thaliana |
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