Any feedback?
Literature summary for 3.6.4.13 extracted from
- Characterization of RNA helicase CshA and its role in protecting mRNAs and small RNAs of Staphylococcus aureus strain Newman (2016), Infect. Immun., 84, 833-844 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cshA, recombinant expression of N-terminally His-tagged wild-type and truncated mutant enzymes in Escherichia coli strain BL21(pLysS), subcloning in Escherichia coli strain DH5alpha | Staphylococcus aureus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of PCR-generated truncated fragments of cshA, including the ATPase and the helicase domains and ending in a stop codon. Genes more abundant in the wild-type than in the cshA mutant upon MazFsa expression, overview. Mutation of cshA affects growth and cell viability | Staphylococcus aureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Staphylococcus aureus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Staphylococcus aureus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Staphylococcus aureus | - |
ADP + phosphate | - |
? | |
| ATP + H2O | Staphylococcus aureus N315 | - |
ADP + phosphate | - |
? | |
| ATP + H2O | Staphylococcus aureus Newman | - |
ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | A0A0H3K9R1 | - |
- |
| Staphylococcus aureus | Q7A4G0 | - |
- |
| Staphylococcus aureus N315 | Q7A4G0 | - |
- |
| Staphylococcus aureus Newman | A0A0H3K9R1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and truncated mutant enzymes from Escherichia coli strain BL21(pLysS) by nickel affinity chromatography and dialysis | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Staphylococcus aureus | ADP + phosphate | - |
? | |
| ATP + H2O | - |
Staphylococcus aureus N315 | ADP + phosphate | - |
? | |
| ATP + H2O | - |
Staphylococcus aureus Newman | ADP + phosphate | - |
? | |
| additional information | purified CshA exhibits typical RNA helicase activities, as exemplified by RNA-dependent ATPase activity and unwinding of the DNA-RNA duplex. Unlabeled duplex DNA oligonucleotide is used as helicase substrate, molecular dynamics, overview | Staphylococcus aureus | ? | - |
- |
|
| additional information | purified CshA exhibits typical RNA helicase activities, as exemplified by RNA-dependent ATPase activity and unwinding of the DNA-RNA duplex. Unlabeled duplex DNA oligonucleotide is used as helicase substrate, molecular dynamics, overview. Recombinant N-terminal His-tagged CshA (gene SA1885, N315 genome) binds to 375-nt sarA mRNA | Staphylococcus aureus | ? | - |
- |
|
| additional information | purified CshA exhibits typical RNA helicase activities, as exemplified by RNA-dependent ATPase activity and unwinding of the DNA-RNA duplex. Unlabeled duplex DNA oligonucleotide is used as helicase substrate, molecular dynamics, overview. Recombinant N-terminal His-tagged CshA (gene SA1885, N315 genome) binds to 375-nt sarA mRNA | Staphylococcus aureus N315 | ? | - |
- |
|
| additional information | purified CshA exhibits typical RNA helicase activities, as exemplified by RNA-dependent ATPase activity and unwinding of the DNA-RNA duplex. Unlabeled duplex DNA oligonucleotide is used as helicase substrate, molecular dynamics, overview | Staphylococcus aureus Newman | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56941, sequence calculation | Staphylococcus aureus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CshA | - |
Staphylococcus aureus |
| DEAD box RNA helicase | - |
Staphylococcus aureus |
| DEAD-box ATP-dependent RNA helicase CshA | UniProt | Staphylococcus aureus |
| NWMN_1985 | - |
Staphylococcus aureus |
| RNA helicase CshA | - |
Staphylococcus aureus |
| SA1885 | - |
Staphylococcus aureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Staphylococcus aureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Staphylococcus aureus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Staphylococcus aureus | sequence calculation | - |
9.49 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | a severe growth defect is observed in the cshA mutant compared with the parent when grown at 25°C but not at 37°C. Activation of MazFsa in the cshA mutant results in lower CFU per milliliter accompanied by a precipitous drop in viability (about 40%) compared to those of the parent and complemented strains. NanoString analysis reveals diminished expression of a small number of mRNAs and 22 small RNAs (sRNAs) in the cshA mutant versus the parent upon MazFsa induction, thus implying protection of these RNAs by CshA. In the case of the sRNA teg049 within the sarA locus, the protective effect is likely due to transcript stability as revealed by reduced half-life in the cshA mutant versus the parent. Mutation of cshA affects growth and cell viability | Staphylococcus aureus |
| metabolism | the toxin MazFsa in Staphylococcus aureus is a sequence-specific endoribonuclease that cleaves the majority of the mRNAs in vivo but spares many essential mRNAs (e.g., secY mRNA) and, surprisingly, an mRNA encoding a regulatory protein (i.e., sarA mRNA). CshA likely stabilizes selective mRNAs and sRNAs in vivo and as a result enhances Staphylococcus aureus survival upon MazFsa induction during stress | Staphylococcus aureus |
| additional information | mutational analysis shows that only the C-terminus of the RNA helicase CshA, representing the highly variable region of the molecule, is necessary for binding to sarA mRNA | Staphylococcus aureus |
| physiological function | CshA likely stabilizes selective mRNAs and sRNAs in vivo and as a result enhances Staphylococcus aureus survival upon MazFsa induction during stress | Staphylococcus aureus |
| physiological function | CshA likely stabilizes selective mRNAs and sRNAs in vivo and as a result enhances Staphylococcus aureus survival upon MazFsa induction during stress. CshA protects sarA mRNA but not spa mRNA in vivo. The enzyme is a DEAD box RNA helicase, an enzyme with distinct helicase and ATPase domains that unwinds double-stranded RNA in an ATP-dependent manner and possesses ATPase activity | Staphylococcus aureus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
