Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Homo sapiens | - |
ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q08211 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme by nickel affinity chromatography and anion exchange chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Homo sapiens | ADP + phosphate | - |
? | |
additional information | substrate determinants for unwinding activity of the DExH/D-Box protein RNA helicase A, overview. RHA translocates efficiently along the 3' overhang of RNA, but not DNA, with a requirement of covalent continuity. Ribose-phosphate backbone lesions on both strands of the nucleic acids, especially on the 3' overhang of the loading strand, affect RHA unwinding significantly. RHA requires RNA on the 3' overhang which directly or indirectly connects with the duplex region to mediate productive unwinding | Homo sapiens | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
DExH/D-Box protein RNA helicase A | - |
Homo sapiens |
RHA | - |
Homo sapiens |
RNA helicase A | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | RNA helicase A (RHA) as a member of the DExH/D-box subgroup of helicase superfamily II | Homo sapiens |
malfunction | ribose-phosphate backbone lesions on both strands of the nucleic acids, especially on the 3' overhang of the loading strand, affect RHA unwinding significantly | Homo sapiens |
metabolism | RNA helicase A (RHA) is involved in virtually all aspects of RNA metabolism | Homo sapiens |
physiological function | RNA helicase A (RHA) is involved in virtually all aspects of RNA metabolism. It exhibits robust RNA helicase activity in vitro | Homo sapiens |