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Literature summary for 3.6.4.13 extracted from

  • Xie, Q.; Liu, J.; Shan, Y.; Wang, S.; Liu, F.
    Substrate determinants for unwinding activity of the DExH/D-Box protein RNA helicase A (2018), Biochemistry, 57, 6662-6668 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Homo sapiens
-
ADP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q08211
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme by nickel affinity chromatography and anion exchange chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
Homo sapiens ADP + phosphate
-
?
additional information substrate determinants for unwinding activity of the DExH/D-Box protein RNA helicase A, overview. RHA translocates efficiently along the 3' overhang of RNA, but not DNA, with a requirement of covalent continuity. Ribose-phosphate backbone lesions on both strands of the nucleic acids, especially on the 3' overhang of the loading strand, affect RHA unwinding significantly. RHA requires RNA on the 3' overhang which directly or indirectly connects with the duplex region to mediate productive unwinding Homo sapiens ?
-
-

Synonyms

Synonyms Comment Organism
DExH/D-Box protein RNA helicase A
-
Homo sapiens
RHA
-
Homo sapiens
RNA helicase A
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

General Information

General Information Comment Organism
evolution RNA helicase A (RHA) as a member of the DExH/D-box subgroup of helicase superfamily II Homo sapiens
malfunction ribose-phosphate backbone lesions on both strands of the nucleic acids, especially on the 3' overhang of the loading strand, affect RHA unwinding significantly Homo sapiens
metabolism RNA helicase A (RHA) is involved in virtually all aspects of RNA metabolism Homo sapiens
physiological function RNA helicase A (RHA) is involved in virtually all aspects of RNA metabolism. It exhibits robust RNA helicase activity in vitro Homo sapiens