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Literature summary for 3.6.4.13 extracted from
- Structural analysis of the spliceosomal RNA helicase Prp28 from the thermophilic eukaryote Chaetomium thermophilum (2016), Acta crystallogr. Sect. F, 72, 409-416 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta 2 (DE3) cells | Thermochaetoides thermophila |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| enzyme in complex with ADP, using 200 mM NaCl, 20% (w/v) PEG 4000, 100 mM Tris pH 8.0 | Thermochaetoides thermophila |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | contains Mg2+ | Thermochaetoides thermophila |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermochaetoides thermophila | G0SBQ7 | - |
- |
| Thermochaetoides thermophila DSM 1495 | G0SBQ7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| GSH Sepharose chromatography and Superdex 75 gel filtration | Thermochaetoides thermophila |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pre-mRNA-splicing ATP-dependent RNA helicase 28 | - |
Thermochaetoides thermophila |
| Prp28 | - |
Thermochaetoides thermophila |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is a spliceosomal DEAD-box helicase which is involved in two steps of spliceosome assembly. It is required for the formation of the pre-catalytic spliceosome, which is ATP-dependent | Thermochaetoides thermophila |
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