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Literature summary for 3.6.4.13 extracted from

  • Erkizan, H.V.; Schneider, J.A.; Sajwan, K.; Graham, G.T.; Griffin, B.; Chasovskikh, S.; Youbi, S.E.; Kallarakal, A.; Chruszcz, M.; Padmanabhan, R.; Casey, J.L.; Ueren, A.; Toretsky, J.A.
    RNA helicase A activity is inhibited by oncogenic transcription factor EWS-FLI1 (2015), Nucleic Acids Res., 43, 1069-1080.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.13

Cloned(Commentary)

Cloned (Comment) Organism
recombinant His-tagged enzyme expression in Spodoptera frugiperda Sf9 cells using the baculovirus expression system Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EWS-FLI1 the small molecule reduces RHA helicase activity in a dose-dependent and enantiomeric manner without affecting intrinsic ATPase activity, the RHA kinetics indicate a complex model. Only (S)-YK-4-279 reverses the EWS-FLI1 inhibition of RHA helicase activity. YK-4-279 inhibition of RHA binding to EWS-FLI1 alters the RNA binding profile of both proteins. EWS-FLI1 modulates RHA helicase activity causing changes in overall transcriptome processing Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Homo sapiens
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q08211
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Spodoptera frugiperda Sf9 cells by nickel affinity and heparin affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Homo sapiens ADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
RHA
-
 Homo sapiens
RNA helicase A
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

General Information

General Information Comment Organism
evolution RNA helicase A (RHA) is a DExH-box RNA helicase Homo sapiens
physiological function enzyme RHA plays multiple roles in cellular biology, some functions requiring its activity as a helicase while others as a protein scaffold. The oncogenic transcription factor EWS-FLI1 requires RHA to enable Ewing sarcoma oncogenesis and growth, small molecule YK-4-279 disrupts this complex in cells Homo sapiens