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Literature summary for 3.6.4.13 extracted from

  • Bernstein, J.; Patterson, D.N.; Wilson, G.M.; Toth, E.A.
    Characterization of the essential activities of Saccharomyces cerevisiae Mtr4p, a 3'->5' helicase partner of the nuclear exosome (2008), J. Biol. Chem., 283, 4930-4942.
    View publication on PubMed
Search for more literature for 3.6.4.13

Activating Compound

Activating Compound Comment Organism Structure
additional information a 20-bp duplex RNA is ineffective in stimulating the (d)ATPase activity of Mtr4p Saccharomyces cerevisiae
Single-stranded RNA stimulates Saccharomyces cerevisiae
tRNA stimulates Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
K177A mutant enzyme shows no stimulation of ATPase activity by single-stranded RNA Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.39
-
 ATP pH 7.5, 37°C Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Saccharomyces cerevisiae Mtr4p can unwind duplex RNA in the presence of ATP and a single-stranded RNA tail in the 3' to 5' direction ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P47047
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O Mtr4p can unwind duplex RNA in the presence of ATP and a single-stranded RNA tail in the 3' to 5' direction Saccharomyces cerevisiae ADP + phosphate
-
 ?
ATP + H2O ATP and dATP are the preferred nucleotide substrates. In the presence of ATP or dATP Mtr4p unwinds the duplex region of a partial duplex RNA substrate in the 3' to 5' direction. Mtr4p displays a marked preference for binding to poly(A) RNA relative to an oligoribonucleotide of the same length and a random sequence Saccharomyces cerevisiae ADP + phosphate
-
 ?
dATP + H2O ATP and dATP are the preferred nucleotide substrates. In the presence of ATP or dATP Mtr4p unwinds the duplex region of a partial duplex RNA substrate in the 3' to 5' direction. Mtr4p displays a marked preference for binding to poly(A) RNA relative to an oligoribonucleotide of the same length and a random sequence Saccharomyces cerevisiae dADP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
Mtr4p
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
22.2
-
 ATP pH 7.5, 37°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Saccharomyces cerevisiae