Activating Compound | Comment | Organism | Structure |
---|---|---|---|
single-stranded DNA | on single-stranded RNA binding, the NS3 enzyme switches to a catalytic competent state imparted by an inward movement of the P-loop, interdomain closure and a change in the divalent metal coordination shell, providing a structural basis for RNA-stimulated ATP hydrolysis | Dengue virus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Dengue virus |
expression of the His-tagged catalytic domain of the NS3 helicase domain from dengue virus serotype 4 in Escherichia coli strain BL21 | Dengue virus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, crystallization of native enzyme, enzyme in complex with adenylyl imidodiphosphate, enzyme in complex with ADP, enzyme in complex with single-stranded RNA and enzyme in complex with single-stranded RNA and ADP | Dengue virus |
purified recombinant His-tagged catalytic domain of the NS3, hanging drop vapour diffusion method, at 13°C over a well solution containing 0.1M MES, pH 6.5, and 20% PEG 3350, X-ray diffraction structure determination and analysis. Crystals for the AMPPNP complex are obtained by cocrystallization of NS3h at 5 mg/ml with 5 mM MnCl2 and 5 mM AMPPNP using a precipitating solution containing 0.1M MES, pH 6.5, and 10% PEG 3350, at 13°C. Crystals with ADP are obtained by cocrystallization at a concentration of 2.5 mg/ml with 5 mM MnCl2 and 5 mM ADP in 0.1 M Tris-HCl, pH 7.0, and 7.5% PEG 3350 at 23°C, further preparation of ternary complexes,overview | Dengue virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates, binding complexes, overview | Dengue virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dengue virus | - |
- |
- |
Dengue virus | - |
serotype 4 | - |
Purification (Comment) | Organism |
---|---|
- |
Dengue virus |
recombinant His-tagged catalytic domain of the NS3 helicase domain from dengue virus serotype 4 from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration | Dengue virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Dengue virus | ADP + phosphate | - |
? | |
additional information | conformational changes during ATP hydrolysis and RNA unwinding: on ssRNA binding, the NS3 enzyme switches to a catalytic competent state imparted by an inward movement of the P-loop, interdomain closure and a change in the divalent metal coordination shell, providing a structural basis for RNA-stimulated ATP hydrolysis. Determination of enzyme structure-function relationship of enzyme bound to single-stranded RNA, to an ATP analogue, to a transition-state analogue and to ATP hydrolysis products. RNA recognition appears largely sequence independent, reaction mechanism and RNA recognition, overview. RNA-unwinding mechanism, overview | Dengue virus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NS3 helicase | - |
Dengue virus |
NS3 protein | - |
Dengue virus |