Cloned (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
F162A | kcat/KM for ATP is 1% of wild-type value | Saccharomyces cerevisiae |
F162L | kcat/KM for ATP is 25% of wild-type value | Saccharomyces cerevisiae |
Q169A | kcat/KM for ATP is 0.3% of wild-type value | Saccharomyces cerevisiae |
Q169E | kcat/KM for ATP is 0.4% of wild-type value | Saccharomyces cerevisiae |
T166A | kcat/KM for ATP is 37% of wild-type value | Saccharomyces cerevisiae |
T166S | kcat/KM for ATP is 26% of wild-type value | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | competitive | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
ATP | pH 7.5, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
0.51 | - |
ATP | pH 7.5, 30°C, mutant enzyme T166A | Saccharomyces cerevisiae | |
0.66 | - |
ATP | pH 7.5, 30°C, mutant enzyme T166S | Saccharomyces cerevisiae | |
0.78 | - |
ATP | pH 7.5, 30°C, mutant enzyme F162L | Saccharomyces cerevisiae | |
1.1 | - |
ATP | pH 7.5, 30°C, mutant enzyme Q169E | Saccharomyces cerevisiae | |
3.5 | - |
ATP | pH 7.5, 30°C, mutant enzyme F162A | Saccharomyces cerevisiae | |
33 | - |
ATP | pH 7.5, 30°C, mutant enzyme Q169A | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P06634 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | the Q motif regulates ATP binding and hydrolysis, the affinity of the protein for RNA substrates and the helicase activity. At least three different protein conformations that are associated with free, ADP-bound and ATP-bound forms of the protein | Saccharomyces cerevisiae | ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DEAD-box RNA helicase | - |
Saccharomyces cerevisiae |
DED1 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
ATP | pH 7.5, 30°C, mutant enzyme Q169E | Saccharomyces cerevisiae | |
0.3 | - |
ATP | pH 7.5, 30°C, mutant enzyme F162A | Saccharomyces cerevisiae | |
1 | - |
ATP | pH 7.5, 30°C, mutant enzyme Q169A | Saccharomyces cerevisiae | |
1.8 | - |
ATP | pH 7.5, 30°C, mutant enzyme T166S | Saccharomyces cerevisiae | |
2 | - |
ATP | pH 7.5, 30°C, mutant enzyme F162L | Saccharomyces cerevisiae | |
2 | - |
ATP | pH 7.5, 30°C, mutant enzyme T166A | Saccharomyces cerevisiae | |
3.5 | - |
ATP | pH 7.5, 30°C, wild-type enzyme | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
ADP | pH 7.5, 30°C, mutant enzyme T166S | Saccharomyces cerevisiae | |
0.36 | - |
ADP | pH 7.5, 30°C, wild-type enzyme | Saccharomyces cerevisiae | |
0.36 | - |
ADP | pH 7.5, 30°C, mutant enzyme T166A | Saccharomyces cerevisiae | |
3 | - |
ADP | pH 7.5, 30°C, mutant enzyme F162L | Saccharomyces cerevisiae |