Literature summary for 3.6.4.10 extracted from

Mercier, R.; Wolmarans, A.; Schubert, J.; Neuweiler, H.; Johnson, J.; LaPointe, P.
The conserved NxNNWHW motif in Aha-type co-chaperones modulates the kinetics of Hsp90 ATPase stimulation (2019), Nat. Commun., 10, 1273 .

Search for more literature for 3.6.4.10

Activating Compound

Activating Compound	Comment	Organism	Structure
Aha1p	potent stimulator of the enzyme ATPase activity	Saccharomyces cerevisiae
Hch1p	-	Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
S25P	the mutation of Hsp82p impairs ATPase stimulation by Aha1p. The intrinsic ATPase activity of the mutant is comparable to that of wildtype enzyme	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
Sba1p	-	Saccharomyces cerevisiae
Sti1p	-	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.118	-	ATP	Hsp82p, in the presence of Aha1p, at pH 7.2, temperature not specified in the publication	Saccharomyces cerevisiae
0.133	-	ATP	Hsp82p, at pH 7.2, temperature not specified in the publication	Saccharomyces cerevisiae
0.466	-	ATP	Hsp82p, in the presence of Hch1p, at pH 7.2, temperature not specified in the publication	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O	Saccharomyces cerevisiae	-	ADP + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography and Superdex S200 gel filtration	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	-	Saccharomyces cerevisiae	ADP + phosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 90000, SDS-PAGE	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
90 kDa heat shock protein	-	Saccharomyces cerevisiae
Hsp82p	-	Saccharomyces cerevisiae
Hsp90	-	Saccharomyces cerevisiae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)