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Literature summary for 3.6.4.10 extracted from
- The amino-terminal domain of Mycobacterium tuberculosis ClpB protein plays a crucial role in its substrate disaggregation activity (2018), FEBS Open Bio, 8, 1669-1690 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain M15 | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | complete loss of enzyme ATPase activity is seen at 0.5 mM ADP. Under saturating levels of ATP (5 mM), the enzyme loses more than 80% of its ATPase activity upon addition of as low a concentration as 0.25 mM ADP | Mycobacterium tuberculosis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 480000 | - |
N-terminally truncated protein, gel filtration | Mycobacterium tuberculosis |
| 560000 | - |
full-length enzyme, gel filtration | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Mycobacterium tuberculosis | - |
ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Superdex S200 gel filtration | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Mycobacterium tuberculosis | ADP + phosphate | - |
? | |
| additional information | the N-terminal domain is dispensable for oligomerization, ATPase activity and prevention of aggregation activity of the enzyme | Mycobacterium tuberculosis | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | 6 * 80000, N-terminally truncated protein, SDS-PAGE | Mycobacterium tuberculosis |
| homohexamer | 6 * 93000, full-length enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ClpB | - |
Mycobacterium tuberculosis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 59.7 | - |
melting temperature | Mycobacterium tuberculosis |
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Release 2023.1 (January 2023)
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