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BRENDA support

Literature summary for 3.6.4.10 extracted from

  • Krajewska, J.; Arent, Z.; Wieckowski, D.; Zolkiewski, M.; Kedzierska-Mieszkowska, S.
    Immunoreactivity of the AAA+ chaperone ClpB from Leptospira interrogans with sera from Leptospira-infected animals (2016), BMC Microbiol., 16, 151 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Leptospira interrogans

Organism

Organism UniProt Comment Textmining
Leptospira interrogans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex S200 gel filtration Leptospira interrogans

Source Tissue

Source Tissue Comment Organism Textmining

Subunits

Subunits Comment Organism
? x * 100000, His-tagged enzyme, SDS-PAGE Leptospira interrogans
? x * 96325, native enzyme calculated from amino acid sequence Leptospira interrogans
? x * 98489, His-tagged enzyme, calculated from amino acid sequence Leptospira interrogans

Synonyms

Synonyms Comment Organism
AAA+ chaperone
-
Leptospira interrogans
ClpB
-
Leptospira interrogans

General Information

General Information Comment Organism
physiological function the enzyme is essential for bacterial survival under stressful conditions and also during infection. The enzyme activates the host immune system Leptospira interrogans