Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.4.10 extracted from

  • Hu, Y.H.; Dang, W.; Zhang, M.; Sun, L.
    Japanese flounder (Paralichthys olivaceus) Hsp70: adjuvant effect and its dependence on the intrinsic ATPase activity (2012), Fish Shellfish Immunol., 33, 829-834.
    View publication on PubMed
Search for more literature for 3.6.4.10

Cloned(Commentary)

Cloned (Comment) Organism
cloning from spleen cDNA, DNA and amino acid sequence determination and analysis, recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Paralichthys olivaceus

Protein Variants

Protein Variants Comment Organism
additional information a mutant PoHsp70, PoHsp70M, that bears a mutation of the ATPase-associated domain, is completely abolished in activity. Construction of the DNA vaccine plasmids with wild-type and mutant enzymes, pSia10Hsp70 and pSia10Hsp70M. pSia10Hsp70 induces a survival rate that is significantly higher than that induced by pSia10, while pSia10Hsp70M induces a survival rate similar to that induced by pSia10 Paralichthys olivaceus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Paralichthys olivaceus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Paralichthys olivaceus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70100
-
 x * 70100, about, sequence calculation Paralichthys olivaceus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Paralichthys olivaceus
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Paralichthys olivaceus O93240
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Paralichthys olivaceus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Paralichthys olivaceus ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 70100, about, sequence calculation Paralichthys olivaceus
More all Hsp70 molecules possess a conserved architecture that consists of a 44 kDa N-terminal adenine nucleotide-binding domain (NBD), an 18 kDa substrate-binding domain (SBD), and a 10 kDa C-terminal domain (CTD) Paralichthys olivaceus

Synonyms

Synonyms Comment Organism
heat shock protein 70
-
 Paralichthys olivaceus
Hsp70
-
 Paralichthys olivaceus
PoHsp70
-
 Paralichthys olivaceus

pI Value

Organism Comment pI Value Maximum pI Value
Paralichthys olivaceus sequence calculation
-
 5.4

Expression

Organism Comment Expression
Paralichthys olivaceus expression of PoHsp70 is upregulated in a time-dependent manner by vaccination of flounder with a DNA vaccine, pSia10, that expresses a Streptococcus iniae antigen Sia10 up

General Information

General Information Comment Organism
additional information all Hsp70 molecules possess a conserved architecture that consists of a 44 kDa N-terminal adenine nucleotide-binding domain (NBD), which hydrolyzes ATP and thus is also called the ATPase domain, an 18 kDa substrate-binding domain (SBD) that contains a hydrophobic pocket in which direct substrate interaction occurs, and a 10 kDa C-terminal domain (CTD) that facilitates substrate trapping by forming a lid-like structure over the substrate binding pocket. PoHsp70 possesses an ATPase domain (residues 108e433) that contains the conserved residues (D201, S210, and G341) involved in ATP binding Paralichthys olivaceus
physiological function heat shock protein 70 is a molecular chaperone that plays an important role in protein folding and transport. It is also involved in regulation of innate and adaptive immune response. PoHsp70 is an effective adjuvant and that the adjuvanticity of PoHsp70 requires the intrinsic ATPase activity Paralichthys olivaceus