Activating Compound | Comment | Organism | Structure |
---|---|---|---|
geranylgeraniol | presence of peptide NRLLLTG eliminates stimulatory effect | Escherichia coli | |
NRLLLTG | peptide binding to the substrate-binding site. Stimulates ATPase activity, eliminates stimulatory effect of geranylgeraniol | Escherichia coli |
Application | Comment | Organism |
---|---|---|
analysis | high-throughput screening method in 96-well plates for ATPase activity of DnaK. Screening of potential inhibitors | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-[1-[(6-[5-[(benzyloxy)carbonyl]-4-biphenyl-4-yl-6-methyl-2-oxo-3,4-dihydropyrimidin-1(2H)-yl]hexanoyl)(hexyl)amino]-2-[(1E)-butylideneamino]-2-oxoethyl]-2-(carboxymethoxy)benzoic acid | - |
Escherichia coli | |
6-[5-[(benzyloxy)carbonyl]-4-(4-cyclohexylphenyl)-6-methyl-2-oxo-3,4-dihydropyrimidin-1(2H)-yl]hexanoic acid | IC50 value about 0.12-0.2 mM | Escherichia coli | |
6-[5-[(benzyloxy)carbonyl]-4-biphenyl-4-yl-6-methyl-2-oxo-3,4-dihydropyrimidin-1(2H)-yl]hexanoic acid | IC50 value about 0.12-0.2 mM | Escherichia coli | |
methyl 4-(naphthalen-2-yl)-2-oxo-6-(phenoxymethyl)-1,2,3,4-tetrahydropyrimidine-5-carboxylate | IC50 value about 0.12 -0.2 mM | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
isoform DnaK | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
high-throughput screening method in 96-well plates for ATPase activity of DnaK. Screening of potential inhibitors | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Escherichia coli | ADP + phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
ATP | pH 7.4, 37°C | Escherichia coli | |
0.0075 | - |
ATP | pH 7.4, 37°C, presence of 0.0009 mM GrpE protein | Escherichia coli | |
0.0083 | - |
ATP | pH 7.4, 37°C, presence of 0.00097 mM DnaJ protein | Escherichia coli | |
0.0125 | - |
ATP | pH 7.4, 37°C, presence of 0.00048 mM DnaJ and 0.0009 mM GrpE proteins | Escherichia coli | |
0.0157 | - |
ATP | pH 7.4, 37°C, presence of 0.00097 mM DnaJ and 0.00091 mM GrpE proteins | Escherichia coli | |
0.167 | - |
ATP | pH 7.4, 37°C, presence of 0.00097 mM DnaJ and 0.0018 mM GrpE proteins | Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.065 | - |
pH 7.4, 37°C, presence of peptide NRLLLTG | Escherichia coli | 5-[1-[(6-[5-[(benzyloxy)carbonyl]-4-biphenyl-4-yl-6-methyl-2-oxo-3,4-dihydropyrimidin-1(2H)-yl]hexanoyl)(hexyl)amino]-2-[(1E)-butylideneamino]-2-oxoethyl]-2-(carboxymethoxy)benzoic acid | |
0.13 | - |
pH 7.4, 37°C | Escherichia coli | 5-[1-[(6-[5-[(benzyloxy)carbonyl]-4-biphenyl-4-yl-6-methyl-2-oxo-3,4-dihydropyrimidin-1(2H)-yl]hexanoyl)(hexyl)amino]-2-[(1E)-butylideneamino]-2-oxoethyl]-2-(carboxymethoxy)benzoic acid |