Literature summary for 3.6.4.10 extracted from

Rowlands, M.G.; Newbatt, Y.M.; Prodromou, C.; Pearl, L.H.; Workman, P.; Aherne, W.
High-throughput screening assay for inhibitors of heat-shock protein 90 ATPase activity (2004), Anal. Biochem., 327, 176-183.

Search for more literature for 3.6.4.10

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
17-allyl-amino-17-demethoxygeldanamycin	0.002 mM, 20% inhibition	Saccharomyces cerevisiae
geldanamycin	0.002 mM, 51% inhibition, 0.005 mM, 50% inhibition	Saccharomyces cerevisiae
additional information	2 further inhibitors are found in a screen with 53440 compounds	Saccharomyces cerevisiae
radicicol	0.002 mM, 88% inhibition, 0.0005 mM, 50% inhibition	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.51	-	ATP	37°C, pH 7.4	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O	Saccharomyces cerevisiae	-	ADP + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant Hsp90, metal affinity, gel filtration, ion-exchange chromatography	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	-	Saccharomyces cerevisiae	ADP + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
Hsp90	-	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0078	-	ATP	37°C, pH 7.4	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)