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Literature summary for 3.6.1.B16 extracted from

  • Ogawa, T.; Yoshimura, K.; Miyake, H.; Ishikawa, K.; Ito, D.; Tanabe, N.; Shigeoka, S.
    Molecular characterization of organelle-type Nudix hydrolases in Arabidopsis (2008), Plant Physiol., 148, 1412-1424.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.B16

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activity increases in presence of 1 mM dithiothreitol Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0096
-
 P1,P5-di(adenosine-5')pentaphosphate pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol Arabidopsis thaliana
0.0218
-
 P1,P4-bis(5'-adenosyl) tetraphosphate pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9CAF2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
P1,P4-bis(5'-adenosyl) tetraphosphate + H2O
-
 Arabidopsis thaliana ?
-
 ?
P1,P5-di(adenosine-5')pentaphosphate + H2O
-
 Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
Nudix hydrolase
-
 Arabidopsis thaliana
NUDT26
-
 Arabidopsis thaliana
NUDX26
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.8
-
 P1,P5-di(adenosine-5')pentaphosphate pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol Arabidopsis thaliana
16.9
-
 P1,P4-bis(5'-adenosyl) tetraphosphate pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function T-DNA insertion mutants do not exhibit any phenotypical differences under normal growth conditions. The gene NUDX26 cannot complement the mutator phenotype of the Escherichia coli mutT- strain Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
770
-
 AppppA pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol Arabidopsis thaliana
1200
-
 ApppppA pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol Arabidopsis thaliana