Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.1.9 extracted from

  • Lin, S.; McLennan, A.G.; Ying, K.; Wang, Z.; Gu, S.; Jin, H.; Wu, C.; Liu, W.; Yuan, Y.; Tang, R.; Xie, Y.; Mao, Y.
    Cloning, expression, and characterization of a human inosine triphosphate pyrophosphatase encoded by the itpa gene (2001), J. Biol. Chem., 276, 18695-18701 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
dITP pH 8.5, 37°C Homo sapiens
0.51
-
ITP pH 8.5, 37°C Homo sapiens
0.57
-
xanthosine 5'-triphosphate pH 8.5, 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activity increases sharply up to about 10 mM MgCl2 and reaches a plateau between about 30 and 100 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9BY32
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland
-
Homo sapiens
-
heart
-
Homo sapiens
-
liver
-
Homo sapiens
-
thyroid gland
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dITP + H2O at the optimal pH of 10.0, the recombinant enzyme hydrolyzes ITP, dITP, and xanthosine 5'-triphosphate to their respective monophosphates whereas activity with other nucleoside triphosphates is low. UTP, (d)CTP, TTP, and (d)ATP are hydrolyzed 10-100fold less than dITP. IDP and IMP are not hydrolyzed at all Homo sapiens dIMP + diphosphate
-
?
ITP + H2O at the optimal pH of 10.0, the recombinant enzyme hydrolyzes ITP, dITP, and xanthosine 5'-triphosphate to their respective monophosphates whereas activity with other nucleoside triphosphates is low. UTP, (d)CTP, TTP, and (d)ATP are hydrolyzed 10-100fold less than dITP. IDP and IMP are not hydrolyzed at all Homo sapiens IMP + diphosphate
-
?
xanthosine 5'-triphosphate + H2O at the optimal pH of 10.0, the recombinant enzyme hydrolyzes ITP, dITP, and xanthosine 5'-triphosphate to their respective monophosphates whereas activity with other nucleoside triphosphates is low. UTP, (d)CTP, TTP, and (d)ATP are hydrolyzed 10-100fold less than dITP. IDP and IMP are not hydrolyzed at all Homo sapiens xanthosine 5'-monophosphate + diphosphate
-
?

Subunits

Subunits Comment Organism
? x * 25000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
inosine triphosphate pyrophosphatase
-
Homo sapiens
ITPase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
360
-
dITP pH 8.5, 37°C Homo sapiens
580
-
ITP pH 8.5, 37°C Homo sapiens
640
-
xanthosine 5'-triphosphate pH 8.5, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
in glycine buffer Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1120
-
xanthosine 5'-triphosphate pH 8.5, 37°C Homo sapiens
1140
-
ITP pH 8.5, 37°C Homo sapiens
1160
-
dITP pH 8.5, 37°C Homo sapiens