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Literature summary for 3.6.1.9 extracted from
- Thermotoga maritima MazG protein has both nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase activities (2003), J. Biol. Chem., 278, 21408-21414 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermotoga maritima |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E41Q/E42Q | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increase in the pyrophosphatase activity | Thermotoga maritima |
| E45Q | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increase in the pyrophosphatase activity | Thermotoga maritima |
| E61Q | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increase in the pyrophosphatase activity | Thermotoga maritima |
| K118A | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increase in the pyrophosphatase activity | Thermotoga maritima |
| R97A/R98A | significant decrease in the nucleoside triphosphate pyrophosphohydrolase activity and concomitant increase in the pyrophosphatase activity | Thermotoga maritima |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha,beta-methyleneadenosine triphosphate | cannot be hydrolyzed and blocks both NTPase and pyrophosphatase activities | Thermotoga maritima |  |
| diphosphate | - |
Thermotoga maritima | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
CTP | pH 8.0, 70°C | Thermotoga maritima | |
| 1 | - |
ATP | pH 8.0, 70°C | Thermotoga maritima | |
| 1 | - |
GTP | pH 8.0, 70°C | Thermotoga maritima | |
| 1 | - |
dGTP | pH 8.0, 70°C | Thermotoga maritima | |
| 1.1 | - |
UTP | pH 8.0, 70°C | Thermotoga maritima | |
| 1.5 | - |
dTTP | pH 8.0, 70°C | Thermotoga maritima | |
| 1.7 | - |
dCTP | pH 8.0, 70°C | Thermotoga maritima | |
| 3.2 | - |
dATP | pH 8.0, 70°C | Thermotoga maritima | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29728 | - |
mass spectrometry | Thermotoga maritima |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | Q9X015 | - |
- |
| Thermotoga maritima DSM 3109 | Q9X015 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Thermotoga maritima | AMP + diphosphate | - |
? | |
| CTP + H2O | - |
Thermotoga maritima | CMP + diphosphate | - |
? | |
| dATP + H2O | - |
Thermotoga maritima | dAMP + diphosphate | - |
? | |
| dCTP + H2O | - |
Thermotoga maritima | dCMP + diphosphate | - |
? | |
| dCTP + H2O | - |
Thermotoga maritima DSM 3109 | dCMP + diphosphate | - |
? | |
| dGTP + H2O | dGTP is the best substrate among the deoxyribonucleoside triphosphates | Thermotoga maritima | dGMP + diphosphate | - |
? | |
| dTTP + H2O | - |
Thermotoga maritima | dTMP + diphosphate | - |
? | |
| GTP + H2O | GTP is the best substrate among the ribonucleoside triphosphates | Thermotoga maritima | GMP + diphosphate | - |
? | |
| additional information | the product of the TM0913 gene, has both nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase activities | Thermotoga maritima | ? | - |
? | |
| additional information | the product of the TM0913 gene, has both nucleoside triphosphate pyrophosphohydrolase and pyrophosphatase activities | Thermotoga maritima DSM 3109 | ? | - |
? | |
| UTP + H2O | - |
Thermotoga maritima | UMP + diphosphate | - |
? | |
| UTP + H2O | - |
Thermotoga maritima DSM 3109 | UMP + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MazG | - |
Thermotoga maritima |
| nucleoside triphosphate pyrophosphohydrolase | - |
Thermotoga maritima |
| TM0913 | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Thermotoga maritima |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 90 | 50°C: about 45% of maximal activity, 90°C: about 95% of maximal activity | Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
stable at | Thermotoga maritima |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
CTP | pH 8.0, 70°C | Thermotoga maritima | |
| 0.18 | - |
UTP | pH 8.0, 70°C | Thermotoga maritima | |
| 0.197 | - |
ATP | pH 8.0, 70°C | Thermotoga maritima | |
| 0.65 | - |
dTTP | pH 8.0, 70°C | Thermotoga maritima | |
| 0.69 | - |
GTP | pH 8.0, 70°C | Thermotoga maritima | |
| 0.94 | - |
dGTP | pH 8.0, 70°C | Thermotoga maritima | |
| 0.99 | - |
dCTP | pH 8.0, 70°C | Thermotoga maritima | |
| 1.12 | - |
dATP | pH 8.0, 70°C | Thermotoga maritima | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
UTP | pH 8.0, 70°C | Thermotoga maritima | |
| 0.198 | - |
ATP | pH 8.0, 70°C | Thermotoga maritima | |
| 0.298 | - |
CTP | pH 8.0, 70°C | Thermotoga maritima | |
| 0.35 | - |
dATP | pH 8.0, 70°C | Thermotoga maritima | |
| 0.44 | - |
dTTP | pH 8.0, 70°C | Thermotoga maritima | |
| 0.57 | - |
dCTP | pH 8.0, 70°C | Thermotoga maritima | |
| 0.68 | - |
GTP | pH 8.0, 70°C | Thermotoga maritima | |
| 0.94 | - |
dGTP | pH 8.0, 70°C | Thermotoga maritima | |
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