Literature summary for 3.6.1.9 extracted from

Gijsbers, R.; Ceulemans, H.; Stalmans, W.; Bollen, M.
Structural and catalytic similarities between nucleotide pyrophosphatases/phosphodiesterases and alkaline phosphatases (2001), J. Biol. Chem., 276, 1361-1368.

Search for more literature for 3.6.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
mutants expressed in COS-1 cells	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
D200N	mutation abolishes formation of the nucleotidylated intermediate	Mus musculus
D358N	mutant enzyme can still form nucleotidylated catalytic intermediates but is hampered in the second step of catalysis	Mus musculus
F239A	mutation halves activity, decreased ability to trap the catalytic intermediate	Mus musculus
F239Q	decreased activity and level of covalent intermediate of the mutant may be accounted for by a decreased binding of the substrate	Mus musculus
H362Q	mutant enzyme can still form nucleotidylated catalytic intermediates but is hampered in the second step of catalysis	Mus musculus
H406Q	mutation abolishes formation of the nucleotidylated intermediate	Mus musculus
K237A	mutation halves activity	Mus musculus
T238S	mutant enzyme can still form nucleotidylated catalytic intermediates but is hampered in the second step of catalysis	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	activity can be restored by addition of 2-5 mM ZnCl2 or CaCl2, but only partially by 2-5 mM MgCl2	Mus musculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
CaCl2	2-5 mM restores activity of EDTA-inhibited enzyme	Mus musculus
MgCl2	2-5 mM partially restores activity of EDTA-inhibited enzyme	Mus musculus
ZnCl2	2-5 mM restores activity of EDTA-inhibited enzyme	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	Q9EQG7	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	mutations D358N and H362Q still allow a weak labeling, whereas mutation His517, Asp200, Asp405 and His406 completely abolish autophosphorylation in presence of ATP	Mus musculus

Synonyms

Synonyms	Comment	Organism
NPP1	-	Mus musculus
nucleotide pyrophosphatase	-	Mus musculus

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Release 2023.1 (January 2023)