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Literature summary for 3.6.1.9 extracted from

  • Bischof, E.; Tran-Thi, T.A.; Decker, K.F.A.
    Nucleotide pyrophosphatase of rat liver. A comparative study on the enzymes solubilized and purified from plasma membrane and endoplasmic reticulum (1975), Eur. J. Biochem., 51, 353-361.
    View publication on PubMed
Search for more literature for 3.6.1.9

Inhibitors

Inhibitors Comment Organism Structure
AMP(CH)2PP competitive inhibition, Ki: 25 mM Rattus sp.
AMP-amidate competitive inhibition, Ki: 30 mM Rattus sp.
AMP-PP(S) competitive inhibition, Ki: 33 mM Rattus sp.
EDTA complete inactivation, partially restored by Mg2+, Ca2+ Rattus sp.
UMP competitive inhibition, Ki: 26 mM Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
15
-
 thymidine 5'-monophosphate p-nitrophenyl ester
-
 Rattus sp.
20
-
 NADH
-
 Rattus sp.
24
-
 UDP-glucose
-
 Rattus sp.
28
-
 ATP
-
 Rattus sp.
28
-
 NAD+
-
 Rattus sp.
30
-
 UTP
-
 Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Rattus sp. 5783
-
nucleus
-
 Rattus sp. 5634
-
plasma membrane
-
 Rattus sp. 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for optimal activity Rattus sp.
Mg2+ required for optimal activity Rattus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
137000
-
 1 * 137000, SDS-PAGE Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Rattus sp.
-
 AMP + diphosphate
-
 ?
NAD+ + H2O Rattus sp.
-
 NMN + AMP
-
 ?
NADH + H2O Rattus sp.
-
 NMNH + AMP
-
 ?
UDP-glucose + H2O Rattus sp.
-
 UMP + D-glucose-1-phosphate
-
 ?
UTP + H2O Rattus sp.
-
 UMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Rattus sp.

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, affinity chromatography Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
145 182
-
 Rattus sp.

Storage Stability

Storage Stability Organism
4°C, lyophilized, no loss of activity Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Rattus sp. AMP + diphosphate
-
 ?
bis(p-nitrophenyl)phosphate + H2O
-
 Rattus sp. p-nitrophenol + p-nitrophenylphosphate
-
 ?
NAD+ + H2O
-
 Rattus sp. NMN + AMP
-
 ?
NADH + H2O
-
 Rattus sp. NMNH + AMP
-
 ?
thymidine 5'-monophosphate p-nitrophenyl ester + H2O
-
 Rattus sp. thymidine 5'-monophosphate + p-nitrophenol
-
 ?
UDP-glucose + H2O
-
 Rattus sp. UMP + D-glucose-1-phosphate
-
 ?
UTP + H2O
-
 Rattus sp. UMP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 137000, SDS-PAGE Rattus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.7
-
-
 Rattus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 inactivation below, very stable at alkaline pH Rattus sp.