Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-ADP | inhibition of monomer | Vigna radiata var. radiata | |
5'-AMP | complete inhibition at 1 mM for monomer, dimer, tetramer, only dimer inhibits at 0.01 mM to 25% | Vigna radiata var. radiata | |
ATP | inhibition of monomer | Vigna radiata var. radiata | |
EDTA | complete inhibition, reversal for dimer and tetramer by Zn2+ | Vigna radiata var. radiata | |
Urea | complete inhibition at 4.5 M for monomer, tetramer inactivated at 6 M | Vigna radiata var. radiata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
FAD | value for dimeric form, biphasic | Vigna radiata var. radiata | |
0.5 | - |
FAD | value for monomeric form | Vigna radiata var. radiata | |
0.58 | - |
FAD | value for tetrameric form | Vigna radiata var. radiata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
1 * 33000, p-hydroxymercuribenzoate treated preparation leads to dissociation to an active monomer, SDS-PAGE | Vigna radiata var. radiata |
33000 | - |
2 * 33000, SDS-PAGE, addition of AMP converts dimer to an active tetramer | Vigna radiata var. radiata |
66000 | - |
gel filtration | Vigna radiata var. radiata |
70000 | - |
gel filtration | Vigna radiata var. radiata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
FAD + H2O | Vigna radiata var. radiata | - |
FMN + AMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna radiata var. radiata | - |
mung bean | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, enzymatically active monomer, dimer and tetramer | Vigna radiata var. radiata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FAD + H2O | - |
Vigna radiata var. radiata | FMN + AMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33000, SDS-PAGE, addition of AMP converts dimer to an active tetramer | Vigna radiata var. radiata |
monomer | 1 * 33000, p-hydroxymercuribenzoate treated preparation leads to dissociation to an active monomer, SDS-PAGE | Vigna radiata var. radiata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
monomeric form | Vigna radiata var. radiata |
49 | - |
dimeric, tetrameric forms | Vigna radiata var. radiata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
half-life: monomer 3 min, dimer 20 min, tetramer 15 min | Vigna radiata var. radiata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.4 | - |
- |
Vigna radiata var. radiata |