General Stability | Organism |
---|---|
resistant to freezing and thawing | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
ATP | - |
Glycine max | |
0.11 | - |
UTP | - |
Glycine max | |
0.18 | - |
ADP | - |
Glycine max | |
0.45 | - |
UDP | - |
Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Glycine max | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
8 * 72000, SDS-PAGE | Glycine max |
560000 | - |
sucrose density gradient centrifugation | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | Glycine max | - |
AMP + phosphate | - |
? | |
ADP-glucose + H2O | Glycine max | - |
AMP + alpha-D-glucose 1-phosphate | - |
? | |
ATP + H2O | Glycine max | - |
AMP + diphosphate | - |
? | |
CDP-glucose + H2O | Glycine max | - |
CMP + D-glucose-1-phosphate | - |
? | |
GDP-glucose + H2O | Glycine max | - |
GMP + D-glucose-1-phosphate | - |
? | |
IDP + H2O | Glycine max | - |
IMP + phosphate | - |
? | |
TDP-glucose + H2O | Glycine max | low activity | TMP + D-glucose-1-phosphate | - |
? | |
UDP + H2O | Glycine max | - |
UMP + phosphate | - |
? | |
UDP-glucose + H2O | Glycine max | best substrate of nucleotide-sugars tested | UMP + D-glucose-1-phosphate | - |
? | |
UMP + H2O | Glycine max | very low activity | uridine + phosphate | - |
? | |
UTP + H2O | Glycine max | - |
UMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
soybean | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | highest levels in leaves of defruited plants | Glycine max | - |
Storage Stability | Organism |
---|---|
-80°C, no loss of activity after 2 months | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | - |
Glycine max | AMP + phosphate | - |
? | |
ADP-glucose + H2O | - |
Glycine max | AMP + alpha-D-glucose 1-phosphate | - |
? | |
ADP-glucose + H2O | - |
Glycine max | AMP + alpha-D-glucose-1-phosphate | - |
? | |
ATP + H2O | - |
Glycine max | AMP + diphosphate | - |
? | |
CDP-glucose + H2O | - |
Glycine max | CMP + D-glucose-1-phosphate | - |
? | |
diphosphate + H2O | significant activity | Glycine max | 2 phosphate | - |
? | |
GDP-glucose + H2O | - |
Glycine max | GMP + D-glucose-1-phosphate | - |
? | |
IDP + H2O | - |
Glycine max | IMP + phosphate | - |
? | |
TDP-glucose + H2O | low activity | Glycine max | TMP + D-glucose-1-phosphate | - |
? | |
thymidine 5'-monophosphate p-nitrophenyl ester + H2O | significant phosphodiesterase activity | Glycine max | thymidine 5'-monophosphate + p-nitrophenol | - |
? | |
UDP + H2O | - |
Glycine max | UMP + phosphate | - |
? | |
UDP-glucose + H2O | best substrate of nucleotide-sugars tested | Glycine max | UMP + D-glucose-1-phosphate | - |
? | |
UMP + H2O | very low activity | Glycine max | uridine + phosphate | - |
? | |
UTP + H2O | - |
Glycine max | UMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 72000, SDS-PAGE | Glycine max |
Synonyms | Comment | Organism |
---|---|---|
nucleotide pyrophosphatase/phosphodiesterase-I | - |
Glycine max |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
no activity after 2 min | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5.5 | - |
Glycine max |
7.5 | - |
optimum for phosphodiesterase I activity | Glycine max |