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Literature summary for 3.6.1.9 extracted from

  • Salvucci, M.E.; Crafts-Brandner, S.J.
    Purification and properties of a unique nucleotide pyrophosphatase/phosphodiesterase I that accumulates in soybean leaves in response to fruit removal (1995), Plant Physiol., 108, 1269-1276.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
resistant to freezing and thawing Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
ATP
-
Glycine max
0.11
-
UTP
-
Glycine max
0.18
-
ADP
-
Glycine max
0.45
-
UDP
-
Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Glycine max 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
8 * 72000, SDS-PAGE Glycine max
560000
-
sucrose density gradient centrifugation Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + H2O Glycine max
-
AMP + phosphate
-
?
ADP-glucose + H2O Glycine max
-
AMP + alpha-D-glucose 1-phosphate
-
?
ATP + H2O Glycine max
-
AMP + diphosphate
-
?
CDP-glucose + H2O Glycine max
-
CMP + D-glucose-1-phosphate
-
?
GDP-glucose + H2O Glycine max
-
GMP + D-glucose-1-phosphate
-
?
IDP + H2O Glycine max
-
IMP + phosphate
-
?
TDP-glucose + H2O Glycine max low activity TMP + D-glucose-1-phosphate
-
?
UDP + H2O Glycine max
-
UMP + phosphate
-
?
UDP-glucose + H2O Glycine max best substrate of nucleotide-sugars tested UMP + D-glucose-1-phosphate
-
?
UMP + H2O Glycine max very low activity uridine + phosphate
-
?
UTP + H2O Glycine max
-
UMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Glycine max
-
soybean
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
leaf highest levels in leaves of defruited plants Glycine max
-

Storage Stability

Storage Stability Organism
-80°C, no loss of activity after 2 months Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O
-
Glycine max AMP + phosphate
-
?
ADP-glucose + H2O
-
Glycine max AMP + alpha-D-glucose 1-phosphate
-
?
ADP-glucose + H2O
-
Glycine max AMP + alpha-D-glucose-1-phosphate
-
?
ATP + H2O
-
Glycine max AMP + diphosphate
-
?
CDP-glucose + H2O
-
Glycine max CMP + D-glucose-1-phosphate
-
?
diphosphate + H2O significant activity Glycine max 2 phosphate
-
?
GDP-glucose + H2O
-
Glycine max GMP + D-glucose-1-phosphate
-
?
IDP + H2O
-
Glycine max IMP + phosphate
-
?
TDP-glucose + H2O low activity Glycine max TMP + D-glucose-1-phosphate
-
?
thymidine 5'-monophosphate p-nitrophenyl ester + H2O significant phosphodiesterase activity Glycine max thymidine 5'-monophosphate + p-nitrophenol
-
?
UDP + H2O
-
Glycine max UMP + phosphate
-
?
UDP-glucose + H2O best substrate of nucleotide-sugars tested Glycine max UMP + D-glucose-1-phosphate
-
?
UMP + H2O very low activity Glycine max uridine + phosphate
-
?
UTP + H2O
-
Glycine max UMP + diphosphate
-
?

Subunits

Subunits Comment Organism
octamer 8 * 72000, SDS-PAGE Glycine max

Synonyms

Synonyms Comment Organism
nucleotide pyrophosphatase/phosphodiesterase-I
-
Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
no activity after 2 min Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5.5
-
Glycine max
7.5
-
optimum for phosphodiesterase I activity Glycine max