Cloned (Comment) | Organism |
---|---|
muscular isoenzyme as fusion protein with glutathione S-transferase, synthetic gene linked to the gene for glutathione S-transferase, expression in Escherichia coli strains DB1035 and TB1 | Homo sapiens |
General Stability | Organism |
---|---|
muscular enzyme, sensitive to urea: urea denaturation | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | phosphate: competitive inhibition, muscular enzyme, native Ki: 2.29 mM, recombinant, expressed as fusion protein with glutathione S-transferase, Ki: 3.05 | Homo sapiens | |
Urea | 2.7 M: 50% inhibition, 8M: 100% inhibition, 90% of its original activity regained in the absence of urea | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data | Homo sapiens | |
0.85 | - |
benzoyl phosphate | muscular enzyme recombinant, expressed as fusion protein with glutathione S-transferase | Homo sapiens | |
1.53 | - |
benzoyl phosphate | muscular enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
11360 | - |
muscle, calculated from amino acid sequence | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
human | - |
Homo sapiens | - |
two different isoenzymes: skeletal muscle and erythrocyte enzyme | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | muscular isoenzyme, acetylated at the N-terminus | Homo sapiens |
Purification (Comment) | Organism |
---|---|
muscular isoenzyme as fusion protein with glutathione S-transferase, subsequently cleaved to fully active acylphosphatase and further purified | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
renaturation after thermal denaturation, 65°C | Homo sapiens |
renaturation after treatment with urea | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4500 | - |
muscular isoenzyme | Homo sapiens |
5000 | - |
muscular isoenzyme, recombinant, expressed as fusion protein with glutathione S-transferase | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-diphosphoglycerate + H2O | - |
Homo sapiens | 3-phosphoglycerate + phosphate | - |
? | |
acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Homo sapiens | carboxylate + phosphate | - |
? | |
benzoyl phosphate + H2O | - |
Homo sapiens | benzoate + phosphate | - |
? | |
carbamoyl phosphate + H2O | - |
Homo sapiens | carbamate + phosphate | - |
? | |
additional information | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Homo sapiens | ? | - |
? | |
additional information | hydrolyzes the beta-aspartylphosphate formed during the action of membrane pumps | Homo sapiens | ? | - |
? | |
succinoyl phosphate + H2O | - |
Homo sapiens | succinate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | two isoenzymatic forms: one prevalent in skeletal and cardiac muscle named muscle type, the other in red blood cells named erythrocyte or common type, even if both isoforms are expressed in all tissues | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
muscular isoenzyme, fully denatured protein. Thermal denaturation is reversibel | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
muscle enzyme is very basic protein | Homo sapiens |
4.7 | 5.6 | muscular enzyme | Homo sapiens |
4.8 | 5.8 | muscular enzyme, recombinant, expressed as fusion protein with glutathione S-transferase | Homo sapiens |
5.3 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2700 | - |
Urea | - |
Homo sapiens |