General Stability | Organism |
---|---|
enzyme very sensitive to 8 M urea | Meleagris gallopavo |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cl- | muscular isoenzyme, Ki: 40.0 mM, erythrocyte isoenzyme, Ki: 31.0 mM | Meleagris gallopavo | |
Phenylglyoxal | 6 mM: inhibition, inhibition partially removed by 35 mM phosphate | Meleagris gallopavo | |
phosphate | muscular isoenzyme, Ki: 2.8 mM, erythrocyte isoenzyme, Ki: 0.68 mM | Meleagris gallopavo | |
Urea | 8 M: 100% inhibition; 8 M: 100% reversibel inhibition, 60% reactivation in the absence of urea | Meleagris gallopavo |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic data | Meleagris gallopavo | |
0.18 | - |
benzoyl phosphate | erythrocyte isoenzyme | Meleagris gallopavo | |
1 | - |
benzoyl phosphate | muscular isoenzyme | Meleagris gallopavo |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12750 | - |
SDS-PAGE | Meleagris gallopavo |
12750 | - |
erythrocyte isoenzyme | Meleagris gallopavo |
13400 | - |
muscular isoenzyme | Meleagris gallopavo |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Meleagris gallopavo | - |
turkey | - |
Meleagris gallopavo | - |
two isoenzymes: muscular isoenzyme, IM, and erythrocyte isoenzyme, IE | - |
Purification (Comment) | Organism |
---|---|
muscle, erythrocyte isoenzyme | Meleagris gallopavo |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an acylphosphate + H2O = a carboxylate + phosphate | thermodynamic data | Meleagris gallopavo |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | skeletal muscle | Meleagris gallopavo | - |
muscle | erythrocyte isoenzyme | Meleagris gallopavo | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3500 | - |
muscular isoenzyme | Meleagris gallopavo |
9000 | - |
erythrocyte isoenzyme | Meleagris gallopavo |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-diphosphoglycerate + H2O | - |
Meleagris gallopavo | 3-phosphoglycerate + phosphate | - |
? | |
acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Meleagris gallopavo | carboxylate + phosphate | - |
? | |
benzoyl phosphate + H2O | - |
Meleagris gallopavo | benzoate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | erythrocyte isoenzyme, 1 * 12750, SDS-PAGE | Meleagris gallopavo |
Synonyms | Comment | Organism |
---|---|---|
More | two isoenzymes in all tissues, organs, and cell types checked: 1. muscular isoenzyme, IM, and 2. erythrocyte isoenzyme, IE, on the basis of the cell-type in which they are mostly represented and from which they are purified for the first time | Meleagris gallopavo |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme is thermostable | Meleagris gallopavo |
additional information | - |
enzyme is thermostable, thermostability is pH-dependent, turkey enzyme is less unstable at higher pH values than horse enzyme | Meleagris gallopavo |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
erythrocyte enzyme is a basic protein | Meleagris gallopavo |
5.3 | - |
muscular and erythrocyte isoenzyme | Meleagris gallopavo |