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Literature summary for 3.6.1.65 extracted from
- Amino acid residues involved in substrate recognition of the Escherichia coli Orf135 protein (2005), Biochemistry, 44, 5683-5689.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D118A | impaired hydrolysis of substrate 2-hydroxy-dATP | Escherichia coli |
| D118E | increase in hydrolysis of substrate 2-hydroxy-dATP | Escherichia coli |
| D118N | impaired hydrolysis of substrate 2-hydroxy-dATP | Escherichia coli |
| E33A | increase in hydrolysis of substrate 2-hydroxy-dATP | Escherichia coli |
| E33Q | increase in hydrolysis of substrate 2-hydroxy-dATP | Escherichia coli |
| R72A | impaired hydrolysis of substrate 2-hydroxy-dATP | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0055 | - |
2-hydroxy-dATP | mutant D118E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |  |
| 0.0057 | - |
5-Methyl-dCTP | mutant D118N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.0069 | - |
5-Methyl-dCTP | mutant D118E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.015 | - |
5-Methyl-dCTP | mutant R72A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.027 | - |
2-hydroxy-dATP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.028 | - |
5-Methyl-dCTP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.067 | - |
2-hydroxy-dATP | mutant E33A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.19 | - |
2-hydroxy-dATP | mutant D118A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.22 | - |
5-Methyl-dCTP | mutant E33A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.24 | - |
5-Methyl-dCTP | mutant D118A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.31 | - |
2-hydroxy-dATP | mutant R72A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.41 | - |
8-hydroxy-dGTP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.65 | - |
dCTP | mutant R72A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.77 | - |
dCTP | mutant D118N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.93 | - |
dCTP | mutant D118E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.99 | - |
dCTP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli JD22899 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hydroxy-dATP + H2O | - |
Escherichia coli | 2-hydroxy-dAMP + diphosphate | - |
? | |
| 2-hydroxy-dATP + H2O | - |
Escherichia coli JD22899 | 2-hydroxy-dAMP + diphosphate | - |
? | |
| 5-methyl-dCTP + H2O | - |
Escherichia coli | 5-methyl-dCMP + diphosphate | - |
? | |
| 5-methyl-dCTP + H2O | - |
Escherichia coli JD22899 | 5-methyl-dCMP + diphosphate | - |
? | |
| 8-hydroxy-dGTP + H2O | - |
Escherichia coli | 8-hydroxy-dGMP + diphosphate | - |
? | |
| 8-hydroxy-dGTP + H2O | - |
Escherichia coli JD22899 | 8-hydroxy-dGMP + diphosphate | - |
? | |
| dCTP + H2O | - |
Escherichia coli | dCMP + diphosphate | - |
? | |
| dCTP + H2O | - |
Escherichia coli JD22899 | dCMP + diphosphate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
2-hydroxy-dATP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.45 | - |
2-hydroxy-dATP | mutant D118E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 0.89 | - |
8-hydroxy-dGTP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.4 | - |
2-hydroxy-dATP | mutant R72A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1.42 | - |
2-hydroxy-dATP | mutant E33A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 2.1 | - |
dCTP | mutant D118E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 2.7 | - |
2-hydroxy-dATP | mutant D118A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 2.7 | - |
dCTP | mutant D118N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 2.8 | - |
5-Methyl-dCTP | mutant E33A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 6.3 | - |
dCTP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 6.5 | - |
5-Methyl-dCTP | mutant D118E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 7.7 | - |
5-Methyl-dCTP | mutant D118N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 8.8 | - |
dCTP | mutant R72A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 9.1 | - |
5-Methyl-dCTP | mutant D118A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 11 | - |
5-Methyl-dCTP | mutant R72A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 35 | - |
5-Methyl-dCTP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.2 | - |
8-hydroxy-dGTP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 2.3 | - |
dCTP | mutant D118E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 3.5 | - |
dCTP | mutant D118N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 4.6 | - |
2-hydroxy-dATP | mutant R72A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 6.4 | - |
dCTP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 6.4 | - |
2-hydroxy-dATP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 13 | - |
5-Methyl-dCTP | mutant E33A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 14 | - |
2-hydroxy-dATP | mutant D118A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 14 | - |
dCTP | mutant R72A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 21 | - |
2-hydroxy-dATP | mutant E33A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 38 | - |
5-Methyl-dCTP | mutant D118A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 82 | - |
2-hydroxy-dATP | mutant D118E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 780 | - |
5-Methyl-dCTP | mutant R72A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 944 | - |
5-Methyl-dCTP | mutant D118E, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1300 | - |
5-Methyl-dCTP | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
| 1400 | - |
5-Methyl-dCTP | mutant D118N, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
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