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Literature summary for 3.6.1.65 extracted from

  • Kamiya, H.; Iida, E.; Harashima, H.
    Important amino acids in the phosphohydrolase module of Escherichia coli Orf135 (2004), Biochem. Biophys. Res. Commun., 323, 1063-1068.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E43A substrate 5-methyl-dCTP, about 20% of wild-type activity, substrate dCTP, 1%of wild-type activity Escherichia coli
E52A complete loss of activity Escherichia coli
E52D complete loss of activity Escherichia coli
E52Q complete loss of activity Escherichia coli
E55A substrate 5-methyl-dCTP, about 20% of wild-type activity, substrate dCTP, 1%of wild-type activity Escherichia coli
E55D substrate 5-methyl-dCTP, about 40% of wild-type activity, substrate dCTP, 2% of wild-type activity Escherichia coli
E55Q substrate 5-methyl-dCTP, about 110% of wild-type activity, substrate dCTP, 10% of wild-type activity Escherichia coli
E56A complete loss of activity Escherichia coli
E56D complete loss of activity Escherichia coli
E56Q complete loss of activity Escherichia coli
G36A complete loss of activity Escherichia coli
G37A complete loss of activity Escherichia coli
K38A substrate 5-methyl-dCTP, about 30% of wild-type activity, substrate dCTP, 10% of wild-type activity Escherichia coli
K38R substrate 5-methyl-dCTP, about 40% of wild-type activity, substrate dCTP, 5% of wild-type activity Escherichia coli
L53A substrate 5-methyl-dCTP, about 30% of wild-type activity, substrate dCTP, 30% of wild-type activity Escherichia coli
R51A substrate 5-methyl-dCTP, about 5% of wild-type activity, substrate dCTP, 1% of wild-type activity Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-dATP + H2O
-
Escherichia coli 2-hydroxy-dAMP + diphosphate
-
?
5-methyl-dCTP + H2O
-
Escherichia coli 5-methyl-dCMP + diphosphate
-
?
8-hydroxy-dGTP + H2O
-
Escherichia coli 8-hydroxy-dGMP + diphosphate
-
?
CTP + H2O
-
Escherichia coli CMP + diphosphate
-
?
dCTP + H2O
-
Escherichia coli dCMP + diphosphate
-
?

Synonyms

Synonyms Comment Organism
Orf135
-
Escherichia coli