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Literature summary for 3.6.1.62 extracted from

  • Tresaugues, L.; Lundbaeck, T.; Welin, M.; Flodin, S.; Nyman, T.; Silvander, C.; Graeslund, S.; Nordlund, P.
    Structural basis for the specificity of human NUDT16 and its regulation by inosine monophosphate (2015), PLoS ONE, 10, e0131507 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.62

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) and Rosetta pLysS cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
apo-form and in complex with IMP and Mg2+, sitting drop vapor diffusion method, using 0.1 MChes pH 9.5 and 20% (w/v) PEG8000 Homo sapiens

Protein Variants

Protein Variants Comment Organism
A22V the differences in the kinetic parameters caused by the A22V mutation are small (less than a factor 2) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
IMP competitive product inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00622
-
 XDP at pH 7.5 and 37°C Homo sapiens
0.00823
-
 ITP at pH 7.5 and 37°C Homo sapiens
0.185
-
 ADP at pH 7.5 and 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-
nucleus
-
 Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ contains two Mg2+ ions Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96DE0
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography, Superdex S200 gel filtration and Superdex S75 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O less efficient substrate Homo sapiens AMP + phosphate
-
 ?
dGDP + H2O less efficient substrate Homo sapiens dGMP + phosphate
-
 ?
dIDP + H2O
-
 Homo sapiens dIMP + phosphate
-
 ?
dITP + H2O less efficient substrate Homo sapiens IDP + phosphate
-
 ?
GDP + H2O less efficient substrate Homo sapiens GMP + phosphate
-
 ?
IDP + H2O
-
 Homo sapiens IMP + phosphate
-
 ?
ITP + H2O less efficient substrate Homo sapiens IDP + phosphate
-
 ?
XDP + H2O less efficient substrate Homo sapiens XMP + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Homo sapiens

Synonyms

Synonyms Comment Organism
Nudt16
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0061
-
 XDP at pH 7.5 and 37°C Homo sapiens
0.0127
-
 ITP at pH 7.5 and 37°C Homo sapiens
0.0959
-
 ADP at pH 7.5 and 37°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0056
-
 IMP with ADP as substrate, at pH 7.5 and 37°C Homo sapiens
0.0098
-
 IMP with ITP as substrate, at pH 7.5 and 37°C Homo sapiens
0.0163
-
 IMP with IDP as substrate, at pH 7.5 and 37°C Homo sapiens
0.0213
-
 IMP with GDP as substrate, at pH 7.5 and 37°C Homo sapiens
0.0352
-
 IMP with XDP as substrate, at pH 7.5 and 37°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.006
-
 with ADP as substrate, at pH 7.5 and 37°C Homo sapiens IMP
0.024
-
 with ITP as substrate, at pH 7.5 and 37°C Homo sapiens IMP
0.103
-
 with XDP as substrate, at pH 7.5 and 37°C Homo sapiens IMP
0.795
-
 with GDP as substrate, at pH 7.5 and 37°C Homo sapiens IMP
3.174
-
 with IDP as substrate, at pH 7.5 and 37°C Homo sapiens IMP

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.52
-
 ADP at pH 7.5 and 37°C Homo sapiens
0.99
-
 XDP at pH 7.5 and 37°C Homo sapiens
1.55
-
 ITP at pH 7.5 and 37°C Homo sapiens