Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.1.6 extracted from

  • Krug, U.; Totzauer, R.; Zebisch, M.; Straeter, N.
    The ATP/ADP substrate specificity switch between Toxoplasma gondii NTPDase1 and NTPDase3 is caused by an altered mode of binding of the substrate base (2013), ChemBioChem, 14, 2292-2300.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with (alpha,beta)-imido-ADP and in the presence of Mg2+ ions, hanging drop vapor diffusion method, using Tris (pH 8.2-8.8, 100 mM), MgCl2 (50 mM), and pentaerythritol propoxylate (17/8 PO/OH, 23-25%, (v/v)) Toxoplasma gondii

Protein Variants

Protein Variants Comment Organism
E493G the mutant of isoform NTPDase1 shows conversion of ATP/ADP specificity compared to the wild type enzyme Toxoplasma gondii
R492G the mutant of isoform NTPDase1 shows conversion of ATP/ADP specificity compared to the wild type enzyme Toxoplasma gondii
R492G/E493G the mutant of isoform NTPDase1 shows conversion of ATP/ADP specificity compared to the wild type enzyme Toxoplasma gondii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.322
-
ADP mutant enzyme E493G, at pH 7.4, temperature not specified in the publication Toxoplasma gondii
0.36
-
ADP mutant enzyme R492G/E493G, at pH 7.4, temperature not specified in the publication Toxoplasma gondii
1.17
-
ADP wild type enzyme, at pH 7.4, temperature not specified in the publication Toxoplasma gondii
2.1
-
ADP mutant enzyme R492G, at pH 7.4, temperature not specified in the publication Toxoplasma gondii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Toxoplasma gondii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + H2O Toxoplasma gondii best substrate AMP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii Q27895
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O best substrate Toxoplasma gondii AMP + phosphate
-
?

Synonyms

Synonyms Comment Organism
NTPDase1
-
Toxoplasma gondii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
93
-
ADP mutant enzyme R492G/E493G, at pH 7.4, temperature not specified in the publication Toxoplasma gondii
109
-
ADP mutant enzyme E493G, at pH 7.4, temperature not specified in the publication Toxoplasma gondii
238
-
ADP wild type enzyme, at pH 7.4, temperature not specified in the publication Toxoplasma gondii
570
-
ADP mutant enzyme R492G, at pH 7.4, temperature not specified in the publication Toxoplasma gondii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
204
-
ADP wild type enzyme, at pH 7.4, temperature not specified in the publication Toxoplasma gondii
254
-
ADP mutant enzyme R492G/E493G, at pH 7.4, temperature not specified in the publication Toxoplasma gondii
270
-
ADP mutant enzyme R492G, at pH 7.4, temperature not specified in the publication Toxoplasma gondii
340
-
ADP mutant enzyme E493G, at pH 7.4, temperature not specified in the publication Toxoplasma gondii