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Literature summary for 3.6.1.6 extracted from

  • Sano, S.; Matsuda, Y.; Nakagawa, H.
    Type B nucleoside-diphosphatase of rat brain. Purification and properties of an enzyme with high thiamin pyrophosphatase activity (1988), Eur. J. Biochem., 171, 231-236.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ADP 1 mM, 27% inhibition of thiamin-diphosphatase activity, competitive Rattus norvegicus
AMP slight competitive inhibition of thiamin-diphosphatase activity Rattus norvegicus
ATP slight competitive inhibition of thiamin-diphosphatase activity Rattus norvegicus
chlorpromazine 1 mM, 15% inhibition Rattus norvegicus
diphosphate complete inhibition at 1 mM, 61% inhibition at 0.06 mM Rattus norvegicus
F- 100 mM KF, 33% inhibition Rattus norvegicus
IMP slight competitive inhibition of thiamin-diphosphatase activity Rattus norvegicus
NEM 1 mM, 16% inhibition Rattus norvegicus
Promethazine 1 mM, 15% inhibition Rattus norvegicus
pyridoxal 5'-phosphate 1 mM, 43% inhibition of thiamin-diphosphatase activity, competitive Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
GDP in presence of Mn2+ Rattus norvegicus
0.54
-
IDP in presence of Mn2+ Rattus norvegicus
0.66
-
thiamine diphosphate in presence of Mn2+ Rattus norvegicus
1.06
-
UDP in presence of Mn2+ Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ in presence of Mn2+ the activity of the enzyme on the substrates is in decreasing order: GDP, TPP, IDP, UDP, CDP Rattus norvegicus
Cd2+ Cd2+ can partially replace Mn2+, 16%, for TPPase activity. For GDPase activity Ca2+ can be partially replaced by Cd2+, 38% Rattus norvegicus
Co2+ Co2+ can partially replace Mn2+, 20%, for TPPase activity. For GDPase activity Ca2+ can be partially replaced by Co2+, 55% Rattus norvegicus
Manganese metalloenzyme contains 0.9 mol zinc and 0.1 mol manganese per mol of 65000 Da subunit Rattus norvegicus
Mg2+ in presence of Mn2+ the activity of the enzyme on the substrates is in decreasing order: GDP, IDP, TPP, UDP, CDP Rattus norvegicus
Mn2+ in presence of Mn2+ the activity of the enzyme on the substrates is in decreasing order: TPP, IDP, GDP, UDP, CDP, dTDP Rattus norvegicus
Zinc metalloenzyme contains 0.9 mol zinc and 0.1 mol manganese per mol of 65000 Da subunit Rattus norvegicus
Zn2+ Zn2+ can partially replace Mn2+, 4%, for TPPase activity. For GDPase activity Ca2+ can be partially replaced by Zn2+, 23% Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
1 * 75000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.43
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O no activity Rattus norvegicus AMP + phosphate
-
?
GDP + H2O
-
Rattus norvegicus GMP + phosphate
-
?
IDP + H2O
-
Rattus norvegicus IMP + phosphate
-
?
TPP + H2O is hydrolyzed twice as efficiently as nucleoside diphosphates Rattus norvegicus thiamine phosphate + phosphate
-
?
UDP + H2O
-
Rattus norvegicus UMP + phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 75000, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
hydrolysis of GDP in presence of Mg2+, TTPase activity in presence of Mg2+ Rattus norvegicus
7
-
GDPase activity in presence of Mn2+ and Ca2+ Rattus norvegicus
7.4
-
hydrolysis of thiamin-diphosphate in presence of Mn2+ Rattus norvegicus
8 8.5 TTPase activity in presence of Ca2+ Rattus norvegicus